文献类型：期刊文献

英文题名：Transposon insertion mutation of Antarctic psychrotrophic fungus for red pigment production adaptive to normal temperature

作者：Ding, Lulu[1];Huang, Hezhou[1];Lu, Fengning[1];Lu, Jian[1];Zhou, Xiangshan[2,3];Zhang, Yuanxing[1,4];Cai, Menghao[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]China Resources Angde Biotech Pharma Co Ltd, 78 E Jiao St, Liaocheng 252299, Shandong, Peoples R China;[3]China Resources Biopharmaceut Co Ltd, 1301-84 Sightseeing Rd, Shenzhen 518110, Peoples R China;[4]Shanghai Collaborat Innovat Ctr Biomfg, Shanghai 200237, Peoples R China

年份：2022

卷号：49

期号：1

外文期刊名：JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

收录：;EI(收录号：20242616385947);WOS:【SCI-EXPANDED(收录号:WOS:000744668300006)】；

基金：This work was supported by the National Key R&D Program of China (Grant number 2018YFC1406706, 2018YFC1406700), National Natural Science Foundation of China (42176238) and the 111 Project of China (Grant number B18022).

语种：英文

外文关键词：Natural red pigment; Antarctic fungus; Geomyces sp.; Protoplast transformation; Transposon mutagenesis

摘要：Polar regions are rich in microbial and product resources. Geomyces sp. WNF-15A is an Antarctic psy chrotrophic filamentous fungus producing high quality red pigment with potential for industrial use. However, efficient biosynthesis of red pigment can only realize at low temperature, which brings difficult control and high cost for the large-scale fermentation. This study aims to develop transposon insertion mutation method to improve cell growth and red pigment production adaptive to normal temperature. Genetic manipulation system of this fungus was firstly developed by antibiotic marker screening, protoplast preparation and transformation optimization, by which transformation efficiency of similar to 50% was finally achieved. Then transposable insertion systems were established using Helitron, Fot1, and Impala transposons. The transposition efficiency reached 11.9%, 9.4%, and 4.6%, respectively. Mutant MP1 achieved the highest red pigment production (OD520 of 39) at 14 degrees C, which was 40% higher than the wild-type strain. Mutant MP14 reached a maximum red pigment production (OD520 of 14.8) at 20 degrees C, which was about twofold of the wild-type strain. Mutants MP2 and MP10 broke the repression mechanism of red pigment biosynthesis in the wild-type and allowed production at 25 degrees C. For cell growth, eight mutants grew remarkably better (12%similar to 30% biomass higher) than the wild-type at 25 degrees C. This study established an efficient genetic manipulation and transposon insertion mutation platform for polar filamentous fungus. It provides reference for genetic breeding of psychrotrophic fungi from polar and other regions.