文献类型：期刊文献

中文题名：应用流式细胞术检测毕赤酵母的细胞活性

英文题名：Application of Flow Cytometry in Viability Detection of Pichia pastoris Cells

作者：肖安风[1];周祥山[1];周利[1];张元兴[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2006

卷号：33

期号：6

起止页码：22

中文期刊名：微生物学通报

外文期刊名：Microbiology China

收录：CSTPCD;;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：流式细胞术;检测;毕赤酵母;细胞活性

外文关键词：Flow cytometry, Detection, Pichia pastoris, Cell viability

摘要：选取两种细胞活性染色试剂二乙酸荧光素(fluoresceindiacetate,FDA)和碘化丙锭(propidiumiodide,PI),应用流式细胞术(flowcytometry,FCM)检测毕赤酵母细胞活性。比较FDA/PI双染色与PI单染色的FCM图谱,后者能够很好地将死活细胞区分开来并得到正确的比例。利用PI单染色检测发酵过程细胞活性的变化,甘油补料阶段几乎没有细胞死亡,进入甲醇补料阶段后,随着细胞密度的增加,细胞的活性不断降低,发酵88h时细胞活性仅为73.8%。
Cell viability of Pichia pastoris was detected by flow cytometry （FCM） with two reagents fluorescein diacetate （FDA） and propidium iodide （PI） . Compared with FDA/PI double-stalned dot plots and PI singlestained dot plots, the latter could divide dead and living ceils into two separate zones, and get the correct proportion. Then PI single-stained method was used to detect the change of cell viability in Pichia patoris fermentation. At glycerol batch and fed-batch phase, little dead ceils were detected. At methanol fed-batch phase, cell viability decreased when cell weight increased, and was only 73.8% at 88 h.