文献类型：期刊文献

英文题名：Enhancing methanogenesis from long-chain fatty acids (LCFA) and enrichment of novel bacteria with resuscitation-promoting factors

作者：Fang, Bo[1,2,4];Liu, Yi-Fan[1,2,4];Wei, Hao-Xun[1,2,4];Zhou, Lei[1,2,4];Yang, Shi-Zhong[1,2,4];Gu, Ji-Dong[3];Mu, Bo-Zhong[1,2,4]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]Guangdong Technion Israel Inst Technol, Environm Sci & Engn Program, 241 Daxue Rd, Shantou 515063, Guangdong, Peoples R China;[4]East China Univ Sci & Technol, Engn Res Ctr Microbial Enhanced Oil Recovery, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2025

卷号：432

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20252018419783);WOS:【SCI-EXPANDED(收录号:WOS:001492016200001)】；

语种：英文

外文关键词：Stimulants; Long-chain fatty acids; Resuscitation promoting factor; Palmitate; Methane production

摘要：Long-chain fatty acids (LCFA) are important intermediate metabolites in lipid hydrolysis during anaerobic digestion for biogas production. High LCFA loads inhibit microbial activity by toxicity, impairing the coupling of beta-oxidation and methanogenesis, thus reducing LCFA degradation efficiency. This study employed and tested seven stimulants, including the resuscitation-promoting factors (Rpf and YeaZ), the quorum-sensing molecules (cAMP, and AHLs), the chemical stimulants (pyruvate), the growth promoter (fumarate), and yeast extract + peptone (YP) for enhancement of methanogenic degradation of LCFA. The results indicate that the chemical stimulants and resuscitation-promoting factors enhanced maximum methane-production rate 1.58 to 2.20 fold versus the NS, reducing the lag phase by 1.46-9.76 days. Analysis of the microbial community composition revealed that the quorum sensing factors only increased species richness, while Rpf, YeaZ fumarate, and YP stimulated the growth of core members of the communities. Metagenomic analysis detected three previously unreported LCFA-degrading bacterial taxa, Marinisomatota, Thermoanaerobaculaceae and Pelomonas. Particularly, Rpf and YeaZ significantly enriched LCFA-degrading bacteria such as Syntrophomonadaceae, Leptospiraceae, and Marine Group B within the core species, while YeaZ also stimulated methanogenic bacteria, possibly due to resuscitating dormant microbes from unfavorable conditions. Syntrophic interactions between LCFA degraders and non-degraders, rather than methanogen abundance, govern methanogenic LCFA degradation. These results demonstrate that the use of stimulants is an effective approach to enhance LCFA degradation and provide a new pathway for energy recovery.