文献类型：期刊文献

中文题名：反胶束液－液萃取牛血清白蛋白的动力学研究II．反萃过程

英文题名：Kinetics of the Solubilization Using Reversed Micelles II.Reextraction of Protein

作者：陆强[1];李宽宏[1];施亚钧[1]

机构：[1]华东理工大学化学工程研究所

年份：1994

卷号：20

期号：6

起止页码：730

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;Scopus;CSCD:【CSCD2011_2012】；

基金：国家教委博士点基金

语种：中文

中文关键词：牛;血清;清蛋白;反胶束萃取;反萃取;动力学

外文关键词：reversed micelles;reextraction;BSA;kinetics;coalescence

摘要：在恒界面池中，从十六烷基三甲基溴化铵反胶束溶液中反萃牛血清白蛋白（ＢＳＡ）的传质系数与搅拌转速无关，与反萃液的ｐＨ值和离子强度有一定关系，而温度的影响强烈（表观活化能达１９６ｋＪ／ｍｏｌ）。表明该反萃过程属界面控制。提出了反萃ＢＳＡ的界面过程机理，“满胶束”的聚结是速率控制步骤，而蛋白质与其周围带电的表面活性剂层的作用，对聚结速率有很大影响。
The overall masstransfer coefficients for reextraction of BSA out of CTAB-reversed micelles were measured using a stirred diffusion cell. They are unaffected by the stirring speed,associated with the pH value and ionic strength in stripping solution,and strong-ly influenced by the operative temperature with a formal activation energy 1 96 kJ/mol·These results showed that the reextraction is controlled by interfacial process.A mechanism of interfacial transport process in the reextraction of BSA is suggested.The coalescence of filled micelles with the phase interface is the rate-limiting step in the reextraction. However,the protein interaction with the surrounding charged surfactant layer has a very strong effect on the coalescence process.