文献类型：期刊文献

英文题名：A qualitative and quantitative high-throughput assay for screening of gluconate high-yield strains by Aspergillus niger

作者：Shi, Fei[1];Tan, Jun[1,2];Chu, Ju[1,2];Wang, Yonghong[1,2];Zhuang, Yingping[1,2];Zhang, Siliang[1,2]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Collaborat Innovat Ctr Biomafg Technol, Shanghai 200237, Peoples R China

年份：2015

卷号：109

起止页码：134

外文期刊名：JOURNAL OF MICROBIOLOGICAL METHODS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000349585900021)】；

基金：This work was financially supported by the Major State Basic Research Development Program of China (973 Program), No. 2013CB733600, a grant from The National Natural Science Foundation of China (21276081), and the National Major Scientific and Technological Special Project for "Significant Scientific Instrument and Equipment Development" (2012YQ15008709).

语种：英文

外文关键词：Aspergillus niger; Gluconate production; Colorimetric; High-throughput screening

摘要：A novel two-step high-throughput strategy was developed for screening of gluconate high-yield strains by Aspergillus niger. The first step was fast qualitative assay according to the indicator color change, the second step was quantitative assay according to the absorbance of chelate formed with Cu2+ at 810 nm. The accuracy of high-throughput assay was comparable to that of high-performance liquid chromatography (HPLC). The correlation coefficient between CuSO4 assay and HPLC assays was exceeding 0.99 by statistical analysis. As a result, 3 high-yield mutants were screened out from 1000 viable single colonies, the mutants II-2-A1, IV-7-C6, and V-11-05 were further validated in 5 L of bioreactor. The average production rates were 15.5%, 32.8%, and 12.1% higher than that of the parental strain, respectively. (C) 2014 Elsevier B.V. All rights reserved.