文献类型：期刊文献

英文题名：Development of a simple and high-yielding fed-batch process for the production of porcine circovirus type 2 virus-like particle subunit vaccine

作者：Cao, Wenlong[1];Cao, Hui[2];Yi, Xiaoping[1];Zhuang, Yingping[1]

机构：[1]East China Univ Sci & Technol, Sch Biotechnol, Shanghai 200237, Peoples R China;[2]Zhejiang EBVAC Biotech Co Ltd, Hangzhou 310018, Zhejiang, Peoples R China

年份：2019

卷号：9

期号：1

外文期刊名：AMB EXPRESS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000489701300004)】；

基金：The Shanghai Zhangjiang National Innovation Demonstration Zone Key projects of Special Development Funds (201310-FX-B2-002) and the Chinese National Programs for High Technology Research and Development (2015AA020801).

语种：英文

外文关键词：Virus-like particles; Porcine circovirus; Subunit vaccine; Fed-batch process; Baculoviral expression system; Feeding "cocktail"

摘要：The cap protein is encoded by the orf2 gene of porcine circovirus type 2 (PCV2) has the main antigen epitope of PCV2 and can form virus-like particles (VLPs), which are expressed in insect cells. PCV2-VLPs can effectively inhibit PCV2 replication as a subunit vaccine. In this study, a robust and reliable fed-batch process was successfully developed for the production of PCV2-VLPs by Sf9 cells. The feeding solution, feeding strategy, and cell density at infection were optimized to maximize the final PCV2-VLPs production yields. The cell density at infection and the volumetric PCV2-VLPs production reached 12 x 10(6) cells/mL and 110 mg/L, respectively, which yielded 3- and 3.6-fold enhancements compared to the batch culture. The PCV2-VLPs produced in fed-batch culture were not different from the PCV2-VLPs produced in a batch culture in an immunity test. A highly efficient production process was produced for PCV2-VLPs subunit vaccines, which could provide an effective means for the industrial production of PCV2 vaccines.