文献类型：期刊文献

英文题名：Ti4+-immobilized multilayer polysaccharide coated magnetic nanoparticles for highly selective enrichment of phosphopeptides

作者：Xiong, Zhichao[1,2];Zhang, Lingyi[1];Fang, Chunli[2];Zhang, Quanqing[2];Ji, Yongsheng[2,3];Zhang, Zhang[2];Zhang, Weibing[1,2];Zou, Hanfa[2]

机构：[1]E China Univ Sci & Technol, Dept Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 1160237, Peoples R China;[3]Henan Univ Tradit Chinese Med, Coll Pharm, Zhengzhou 450000, Peoples R China

年份：2014

卷号：2

期号：28

起止页码：4473

外文期刊名：JOURNAL OF MATERIALS CHEMISTRY B

收录：;EI(收录号：20142717902734);WOS:【SCI-EXPANDED(收录号:WOS:000338646000010)】；

基金：Financial support is gratefully acknowledged from the National Natural Sciences Foundation of China (21235006), the Science and Technology Commission of Shanghai Municipality (12JG0500200), the Creative Research Group Project by NSFC (2132106), the National Key Scientific Instrument and Equipment Development Project (2012YQ120044), the China State Key Basic Research Program Grant (2013CB911202, 2012CB-910601 and 2012CB-910101), the Analytical Method Innovation Program of MOST (2012IM030900) and the Knowledge Innovation program of DICP to H. F. Zou.

语种：英文

外文关键词：Proteins - Synthesis (chemical) - Titanium compounds - Magnetic separation - Magnetite - Silica nanoparticles - Affinity chromatography - Hydrophilicity - Multilayers - Nanomagnetics

摘要：Highly selective and efficient enrichment of trace phosphorylated proteins or peptides from complex biological samples is of profound significance for the discovery of disease biomarkers in biological systems. In this study, a novel immobilized metal affinity chromatography (IMAC) material has been synthesized to improve the enrichment specificity and sensitivity for phosphopeptides by introducing a titanium phosphate moiety on a multilayer polysaccharide (hyaluronate (HA) and chitosan (CS)) coated Fe3O4@SiO2 nanoparticle (denoted as Fe3O4@ SiO2@(HA/CS)(10)-Ti4+ IMAC). The thicker multilayer polysaccharide endows excellent hydrophilic properties and a higher binding capacity of the titanium ion to the IMAC material. Due to the combination of uniform magnetic properties, highly hydrophilic properties and enhanced binding capacity of the titanium ion, the Fe3O4@SiO2@(HA/CS)(10)-Ti4+ nanoparticle possesses many merits, such as high selectivity for phosphopeptides (phosphopeptides/non-phosphopeptides at a molar ratio of 1 : 2000), extreme detection sensitivity (0.5 fmol), large binding capacity (100 mg g(-1)) high enrichment recovery (85.45%) and rapid magnetic separation (within 10 s). Moreover, the as- prepared IMAC nanoparticle provides effective enrichment of phosphopeptides from real samples (human serum and nonfat milk), showing great potential as a tool for the detection and identification of low-abundance phosphopeptides in biological samples.