文献类型：期刊文献

英文题名：Photometric determination of free cholesterol via cholesterol oxidase and carbon nanotube supported Prussian blue as a peroxidase mimic

作者：He, Yanfang[1];Niu, Xiangheng[1];Shi, Libo[2];Zhao, Hongli[2];Li, Xin[1];Zhang, Wenchi[1];Pan, Jianming[1];Zhang, Xifeng[1];Yan, Yongsheng[1];Lan, Minbo[2,3]

机构：[1]Jiangsu Univ, Sch Chem & Chem Engn, Inst Green Chem & Chem Technol, Zhenjiang 212013, Peoples R China;[2]East China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2017

卷号：184

期号：7

起止页码：2181

外文期刊名：MICROCHIMICA ACTA

收录：;EI(收录号：20242616498070);WOS:【SCI-EXPANDED(收录号:WOS:000403353000034)】；

基金：This work was financially supported by the National Natural Science Foundation of China (Nos. 21605061 and 31601549), the Natural Science Foundation of Jiangsu Province (No. BK20160489), the Natural Science Fund for Colleges and Universities in Jiangsu Province (No. 16KJB150009), the Postdoctoral Fund of China (No. 2016 M600365), the Postdoctoral Fund of Jiangsu Province (No. 1601015B), the Open Fund from the Shanghai Key Laboratory of Functional Materials Chemistry (No. SKLFMC201601), the Start-Up Research Fund of Jiangsu University (No. 15JDG143), and the Open Funding Project of the State Key Laboratory of Bioreactor Engineering.

语种：英文

外文关键词：Colorimetric analysis; Peroxidase-like activity; Nanozyme; Hydrogen peroxide; Visualized detection; Hydroxy radical

摘要：The authors describe a photometric method for the determination of free cholesterol based on the oxidation of cholesterol by the catalytic action of the enzyme cholesterol oxidase. The hydrogen peroxide formed is used to oxidize the chromogenic compound 3,3',5,5'-tetramethylbenzidine (TMB) by exploiting the peroxidase-like activity of carbon nanotube-supported Prussian blue (PB). This enzyme mimic was synthesized from a mixture of ferric chloride and hexacyanoferrate(III) in the presence of intrinsically reducing multi-walled carbon nanotubes. The composite can trigger the formation of a blue-green dye from TMB in the presence of H2O2. The findings were exploited to design a photometric (652 nm) assay that has a linear response in the 4 to 100 mu M cholesterol concentration range and a 3 mu M detection limit. The practicability of the method was verified by the successful analysis of free cholesterol in human blood samples.