文献类型：期刊文献

英文题名：Bioprocess exploration for thermostable α-amylase production of a deep-sea thermophile Geobacillus sp in high-temperature bioreactor

作者：Jiang, Tao[1];Huang, Mengmeng[1];He, Hao[1];Lu, Jian[1];Zhou, Xiangshan[1];Cai, Menghao[1];Zhang, Yuanxing[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2016

卷号：46

期号：6

起止页码：620

外文期刊名：PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000382759300012)】；

基金：This work was financially supported by the Chinese National High Technology Research and Development Program (No. 2012AA092103, 2012AA092105).

语种：英文

外文关键词：Geobacillus; high-temperature bioreactor; statistical methodologies; thermophile

摘要：Geobacillus sp. 4j, a deep-sea high-salt thermophile, was found to produce thermostable alpha-amylase. In this work, culture medium and conditions were first optimized to enhance the production of thermostable alpha-amylase by statistical methodologies. The resulting extracellular production was increased by five times and reached 6.40 U/ml. Then, a high-temperature batch culture of the thermophile in a 15 l in-house-designed bioreactor was studied. The results showed that a relatively high dissolved oxygen (600 rpm and 15 l/min) and culture temperature of 60 degrees C facilitated both cell growth and. -amylase production. Thus, an efficient fermentation process was established with initial medium of pH 6.0, culture temperature of 60 degrees C, and dissolved oxygen above 20%. It gave an alpha-amylase production of 79 U/ml and productivity of 19804 U/l.hr, which were 10.8 and 208 times higher than those in shake flask, respectively. This work is useful for deep-sea high-salt thermophile culture, where efforts are lacking presently.