文献类型：期刊文献

英文题名：Photogenerated Aldehydes for Protein Patterns on Hydrogels and Guidance of Cell Behavior

作者：Ming, Zunzhen[1];Fan, Jinyan[1];Bao, Chunyan[1];Xue, Yuan[1];Lin, Qiuning[1];Zhu, Linyong[1]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Inst Fine Chem, Key Lab Adv Mat, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2018

卷号：28

期号：14

外文期刊名：ADVANCED FUNCTIONAL MATERIALS

收录：;EI(收录号：20180604742188);WOS:【SCI-EXPANDED(收录号:WOS:000429410400023)】；

基金：This work was supported by the NSFC (Grant Nos. 21472044, 51273064, 21425311, and 51403061), and the Fundamental Research Funds for the Central Universities (Grant No. 222201717003).

语种：英文

外文关键词：cell behavior; covalent conjugation; hydrogels; postfunctionalization; protein photopatterns

摘要：The immobilization of proteins to hydrogels is important and plays a significant role to provide suitable biomimetic material as extracellular matrix for cell behavior mediation. This study describes a novel and universal strategy for photopatterning unmodified proteins on hydrogels. The methodology creates photogenerated aldehyde regions within a protein-resistant hydrogel and then conjugates unmodified proteins by mild imine ligation with spatial, temporal, and dosage control. The relatively stable aldehyde intermediate enables the facile and highly efficient covalent immobilization of proteins by a postfunctionalization methodology and the sequential protein patterns provide an easy access to control the identity and dynamic change of proteins presented to cells on demand, thus mediating cell behaviors. This approach provides important opportunities for understanding and controlling cell behavior mediated by proteins, and opens up new avenues for hydrogels in tissue engineering and biotechnology applications.