文献类型：期刊文献

英文题名：Enhanced acetoin production by Serratia marcescens H32 with expression of a water-forming NADH oxidase

作者：Sun, Jian-An[1]; Zhang, Liao-Yuan[2]; Rao, Ben[1]; Shen, Ya-Ling[1]; Wei, Dong-Zhi[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Meilong Road 130, Shanghai 200237, China; [2] School of Life Science, FuJian Agriculture and Forestry University, Shangxiadian Road 102, Fuzhou 350002, China

年份：2012

卷号：119

起止页码：94

外文期刊名：Bioresource Technology

收录：EI(收录号：20123015281472)

语种：英文

外文关键词：Batch cell culture - Gene encoding

摘要：Cofactor engineering was employed to enhance production of acetoin by Serratia marcescens H32. 2,3-Butanediol was a major byproduct of acetoin fermentation by S. marcescens H32. In order to decrease 2,3-butanediol formation and achieve a high efficiency of acetoin production, nox gene encoding a water-forming NADH oxidase from Lactobacillus brevis was expressed. Batch fermentations suggested the expression of the NADH oxidase could increase the intracellular NAD+ concentration (1.5-fold) and NAD+/NADH ratio (2.9-fold). Meanwhile, 2,3-butanediol was significantly decreased (52%), and the accumulation of acetoin was enhanced (33%) accordingly. By fed-batch culture of the engineered strain, the final acetoin titer up to 75.2g/l with the productivity of 1.88g/(lh) was obtained. To the best of our knowledge, these results were new records on acetoin fermentation ever reported. ? 2012 Elsevier Ltd.