文献类型：期刊文献

中文题名：γ干扰素免疫电极的研究

英文题名：A Study on the Interferon -γ, Immunnoelectrode

作者：李凡超[1];陈俊[1];张嗣良[1];徐京宁[1]

机构：[1]华东化工学院生化工程研究所

年份：1992

卷号：5

期号：4

起止页码：11

中文期刊名：传感技术学报

外文期刊名：Chinese Journal of Sensors and Actuators

收录：CSTPCD;;Scopus;CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：电极;免疫电极;干扰素;酶

外文关键词：electrode immunnoelectrode interferon-γ enzyme

摘要：用溴化氰在醋酸纤维素膜上固定了马抗人γ干扰素抗体,该抗体膜在37℃下与样品液温育反应1h后,置于一碘离子选择电极端部而构成抗体电极,另一个碘离子选择电极与空白膜组装成参比电极.测量了电极在0.1mmol KI+4.4mmol H_2O_2+0.1mol磷酸盐缓冲液pH5.0中的响应,结果表明电极电位与样品中γ干扰素浓度呈曲线关系.抗体膜可重复使用6d以上.还介绍了过氧化物酶标记的γ干扰素制备方法和电极响应线性化方法.
An antibody membrane was prepared using cyanogen bromide horse antibody against human IFNr covalently immobilized on cellular membrane. After incubated at 37℃ for one hour in sample solution, the membrane was put on the end of an iodide selective electrode to form an antibody elect-. rode. Another iodide selective electrode assembled with a cellular membrane worked as a reference electrode. The electrode response was measured in O.lmmol KI + 4.4mmol H2O2 + 0.lmol phosphate buffer solution pH 5.0. The results showed a curve relation between electrode potential and the IFNr concentration. The antibody membrane can be used repeatly for more than six days. The preparation method of peroxidase-labelled IFNr and the linearization method of electrode response were discribed in this paper.