文献类型：期刊文献

英文题名：Real-time fluid dynamics investigation and physiological response for erythromycin fermentation scale-up from 50 L to 132 m³ fermenter

作者：Zou, Xiang[1,2];Xia, Jian-ye[1];Chu, Ju[1];Zhuang, Ying-ping[1];Zhang, Si-liang[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Southwest Univ, Coll Pharmaceut Sci, Chongqing 400715, Peoples R China

年份：2012

卷号：35

期号：5

起止页码：789

外文期刊名：BIOPROCESS AND BIOSYSTEMS ENGINEERING

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000303533900013)】；

基金：This work was financially supported by a grant from the National Scientific and Technological Major Special Project (Significant Creation of New drugs), No. 2011ZX09203-001-03, and the Major State Basic Research Development Program of China (973 Program), No. 2007CB714303.

语种：英文

外文关键词：Erythromycin biosynthesis; Fermentation; Fluid mechanics; Non-Newtonian fluids; Physiological response; Scale-up

摘要：The physiological response of erythromycin fermentation scale-up from 50 L to 132 m(3) scale was investigated. A relatively high oxygen uptake rate (OUR) in early phase of fermentation was beneficial for erythromycin biosynthesis. Correspondingly, the maximal consistency coefficient (K) reflecting non-Newtonian fluid characteristics in 50 L and 132 m(3) fermenter also appeared in same phase. Fluid dynamics in different scale bioreactor was further investigated by real-time computational fluid dynamics modeling. The results of simulation showed that the impeller combination in 50 L fermenter could provide more modest flow field environment compared with that in 132 m(3) fermenter. The decrease of oxygen transfer rate (OTR) in 132 m(3) fermenter was the main cause for impairing cell physiological metabolism and erythromycin biosynthesis. These results were helpful for understanding the relationship between hydrodynamic environment and physiological response of cells in bioreactor during the scale-up of fermentation process.