文献类型：期刊文献

英文题名：Evolution of mitosome metabolism and invasion-related proteins in Cryptosporidium

作者：Liu, Shiyou[1,2];Roellig, Dawn M.[2];Guo, Yaqiong[1,2];Li, Na[1,2];Frace, Michael A.[3];Tang, Kevin[3];Zhang, Longxian[4];Feng, Yaoyu[1];Xiao, Lihua[2]

机构：[1]East China Univ Sci & Technol, Sch Resources & Environm Engn, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Ctr Dis Control & Prevent, Div Foodborne Waterborne & Environm Dis, Atlanta, GA 30329 USA;[3]Ctr Dis Control & Prevent, Div Sci Resources, Atlanta, GA 30329 USA;[4]Henan Agr Univ, Coll Anim Sci & Vet Med, Zhengzhou 450002, Peoples R China

年份：2016

卷号：17

外文期刊名：BMC GENOMICS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000389843600004)】；

基金：This work was supported by the National Natural Science Foundation of China (31425025, 31229005, 31110103901 and 31302078); Open Funding Project of the State Key Laboratory of Veterinary Etiological Biology, Lanzhou, China (SKLVEB2014KFKT008); and the US Centers for Disease Control and Prevention.

语种：英文

外文关键词：Reductive evolution; Genomics; Mitosome metabolism; Apicomplexa; Cryptosporidium

摘要：Background: The switch from photosynthetic or predatory to parasitic life strategies by apicomplexans is accompanied with a reductive evolution of genomes and losses of metabolic capabilities. Cryptosporidium is an extreme example of reductive evolution among apicomplexans, with losses of both the mitosome genome and many metabolic pathways. Previous observations on reductive evolution were largely based on comparative studies of various groups of apicomplexans. In this study, we sequenced two divergent Cryptosporidium species and conducted a comparative genomic analysis to infer the reductive evolution of metabolic pathways and differential evolution of invasion-related proteins within the Cryptosporidium lineage. Results: In energy metabolism, Cryptosporidium species differ from each other mostly in mitosome metabolic pathways. Compared with C. parvum and C. hominis, C. andersoni possesses more aerobic metabolism and a conventional electron transport chain, whereas C. ubiquitum has further reductions in ubiquinone and polyisprenoid biosynthesis and has lost both the conventional and alternative electron transport systems. For invasion-associated proteins, similar to C. hominis, a reduction in the number of genes encoding secreted MEDLE and insulinase-like proteins in the subtelomeric regions of chromosomes 5 and 6 was also observed in C. ubiquitum and C. andersoni, whereas mucin-type glycoproteins are highly divergent between the gastric C. andersoni and intestinal Cryptosporidium species. Conclusions: Results of the study suggest that rapidly evolving mitosome metabolism and secreted invasion-related proteins could be involved in tissue tropism and host specificity in Cryptosporidium spp. The finding of progressive reduction in mitosome metabolism among Cryptosporidium species improves our knowledge of organelle evolution within apicomplexans.