文献类型：期刊文献

英文题名：Protein Interaction with Zwitterionic Spherical Polyelectrolyte Brushes as Observed by Small Angle X-ray Scattering

作者：Zhang, Ziyu[1];Zhang, Yuhua[1];Liu, Xin[1];Guo, Jiangtao[1];Li, Li[1];Wang, Weihua[2];He, Guofeng[1,3];Guo, Xuhong[1,4]

机构：[1]East China Univ Sci & Technol, Sch Chem Engn, State Key Lab Chem Engn, Shanghai 200237, Peoples R China;[2]SINOPEC Shanghai Res Inst Petrochem Technol, Shanghai 201208, Peoples R China;[3]Jiangsu Feymer Technol Co Ltd, Zhangjiagang 215613, Peoples R China;[4]Chinese Acad Agr Sci, Inst Bast Fiber Crops, Changsha 410205, Peoples R China

年份：2025

卷号：26

期号：4

起止页码：2402

外文期刊名：BIOMACROMOLECULES

收录：;EI(收录号：20251018008908);WOS:【SCI-EXPANDED(收录号:WOS:001437928600001)】；

基金：We thank the staff members of the BL19U2 beamline (https://cstr.cn/31129.02.NFPS.BL19U2) at the National Facility for Protein Science in Shanghai (https://cstr.cn/31129.02.NFPS) for providing technical support and assistance in data collection and analysis. We gratefully thank financial support from the National Key R&D Program of China (2023YFD1700303) and the Science and Technology Innovation Program of Hunan Province (2024RC7001).

语种：英文

外文关键词：Ammonium hydroxide - Grafting (chemical)

摘要：Understanding how zwitterionic spherical polyelectrolyte brushes (SPB) fulfill their antifouling functions requires knowledge of their interactions with exogenous nanoparticles, such as proteins. In this study, zwitterionic SPB were synthesized by grafting 3-[(2-(methacryloyloxy)ethyl)dimethylammonio]propanoate (CBMA) and [2-(methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl) ammonium hydroxide (SBMA) onto a polystyrene core via photoemulsion polymerization. Small-angle X-ray scattering was employed to elucidate the interactions and protein adsorption behaviors of the zwitterionic SPB and proteins. SAXS results revealed that both PCBMA SPB and PSBMA SPB exhibit minimal protein adsorption compared with cationic and anionic SPB. PSBMA SPB maintained a consistent resistance to protein adsorption across various conditions. However, PCBMA SPB demonstrated tunable protein adsorption properties, enabled by the controllable ionization of carboxyl groups on the brush chains while maintaining consistently low overall adsorption. These insights enhance our understanding of zwitterionic SPB and offer an experimental and theoretical basis for their application in biomaterials and antifouling technologies.