文献类型：期刊文献

英文题名：Probing the growth and mechanical properties of Bacillus subtilis biofilms through genetic mutation strategies

作者：Liu, Suying[1,2,3,10];Huang, Jiaofang[4];Zhang, Chen[5];Wang, Lihua[6];Fan, Chunhai[7];Zhong, Chao[3,8,9]

机构：[1]Chinese Acad Sci, Div Phys Biol, Shanghai, Peoples R China;[2]Univ Chinese Acad Sci, Beijing, Peoples R China;[3]ShanghaiTech Univ, Sch Phys Sci & Technol, Shanghai, Peoples R China;[4]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[5]Swiss Fed Inst Technol Lausanne EPFL, Inst Phys, Lausanne, Switzerland;[6]Chinese Acad Sci, Shanghai Adv Res Inst, Interdisciplinary Res Ctr, Zhangjiang Lab, Shanghai, Peoples R China;[7]Shanghai Jiao Tong Univ, Renji Hosp, Inst Mol Med, Sch Chem & Chem Engn,Sch Med, Shanghai, Peoples R China;[8]Chinese Acad Sci, Shenzhen Inst Synthet Biol, Shenzhen Inst Adv Technol, Ctr Mat Synthet Biol, Shenzhen, Peoples R China;[9]Chinese Acad Sci, Shenzhen Inst Synthet Biol, Shenzhen Inst Adv Technol, CAS Key Lab Quantitat Engn Biol, Shenzhen, Peoples R China;[10]Chinese Acad Sci, Shanghai Inst Appl Phys, Bioimaging Ctr, Shanghai, Peoples R China

年份：2022

卷号：7

期号：3

起止页码：965

外文期刊名：SYNTHETIC AND SYSTEMS BIOTECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000822912600002)】；

基金：SEM and TEM characterization was performed at the ShanghaiTech EM Center, Shanghai. This work was partially funded by the National Key R & D Program of China (Nos. 2020YFA0908100 and 2021YFA0910800) , the National Science Fund for Distinguished Young Scholars (No. 32125023) for C. Zhong, and partially supported by grants from the National Natural Science Foundation of China (No.31872728) , the National Science and Technology Major Project of the Ministry of Science and Technology of China (2020YFA0908900) and the Science and Technology Commission of Shanghai Municipality (Nos. 19ZR1477100 and 22ZR1416000) for J. F. Huang.

语种：英文

外文关键词：Bacillus subtilis; Biofilms; Synthetic biology; Living materials; Environmental tolerance

摘要：Bacterial communities form biofilms on various surfaces by synthesizing a cohesive and protective extracellular matrix, and these biofilms protect microorganisms against harsh environmental conditions. Bacillus subtilis is a widely used experimental species, and its biofilms are used as representative models of beneficial biofilms. Specifically, B. subtilis biofilms are known to be rich in extracellular polymeric substances (EPS) and other biopolymers such as DNA and proteins like the amyloid protein TasA and the hydrophobic protein BslA. These materials, which form an interconnected, cohesive, three-dimensional polymer network, provide the mechanical stability of biofilms and mediate their adherence to surfaces among other functional contributions. Here, we explored how genetically-encoded components specifically contribute to regulate the growth status, mechanical properties, and antibiotic resistance of B. subtilis biofilms, thereby establishing a solid empirical basis for understanding how various genetic engineering efforts are likely to affect the structure and function of biofilms. We noted discrete contributions to biofilm morphology, mechanical properties, and survival from major biofilm components such as EPS, TasA and BslA. For example, EPS plays an important role in maintaining the stability of the mechanical properties and the antibiotic resistance of biofilms, whereas BslA has a significant impact on the resolution that can be obtained for printing applications. This work provides a deeper understanding of the internal interactions of biofilm components through systematic genetic manipulations. It thus not only broadens the application prospects of beneficial biofilms, but also serves as the basis of future strategies for targeting and effectively removing harmful biofilms.