文献类型：期刊文献

英文题名：Nanopore-Based Single-Biomolecule Interfaces: From Information to Knowledge

作者：Ying, Yi-Lun[1,2];Long, Yi-Tao[1,2]

机构：[1]Nanjing Univ, Sch Chem & Chem Engn, State Key Lab Analyt Chem Life Sci, Nanjing 210023, Jiangsu, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China

年份：2019

卷号：141

期号：40

起止页码：15720

外文期刊名：JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

收录：;EI(收录号：20194107512370);WOS:【SCI-EXPANDED(收录号:WOS:000490358900001)】；

基金：This research was supported by the National Natural Science Foundation of China (21834001, 21922405 and 61871183) and the Excellent Research Program of Nanjing University (ZYJHO04). Yi-Lun Ying is sponsored by the National Ten Thousand Talent Program for Young Top-notch Talent.

语种：英文

外文关键词：Proteins - DNA sequences - Biomolecules - Spectrum analysis - Gene encoding - Ions - Stochastic systems - Biological systems

摘要：Single-molecule measurements have greatly enhanced our understanding of living systems. Biological systems offer nanopores, a sub-class of membrane proteins, the well-defined confined space for accommodating a single molecule. The biological nanopore acts as a single-biomolecule interface for capturing and identifying a single molecule of interest, and thus it can be used as a single-molecule sensor. In this Perspective, we focus on biological nanopore-based single-biomolecule interfaces for single-biomolecule detection. First, we outline the design of the nanopore-based single-biomolecule interface, which provides rich stochastic information regarding each biomolecule. Next, we highlight future research directions beyond DNA sequencing, including detection of rare species, identification of hidden intermediates, spectral analysis of covalent/noncovalent interactions, and tracing of the dynamic pathways of single-biopolymer behaviors. The concept of a "single-molecule ionic spectrum" is discussed, which may allow mapping of noncovalent interactions at an atomic level in the future. We also discuss the challenges and goals for the future to make this measurement possible for addressing entirely new types of biological questions, which would be an exciting area of future research.