文献类型：期刊文献

英文题名：Efficient bioreductive production of (R)-N-Boc-3-hydroxypiperidine by a carbonyl reductase

作者：Chen, Li-Feng[1];Zhang, Yi-Ping[1];Fan, Hai-Yang[1];Wu, Kai[2];Lin, Jin-Ping[1];Wang, Hua-Lei[1];Wei, Dong-Zhi[1]

机构：[1]East China Univ Sci & Technol, New World Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Univ Med & Hlth Sci, Sch Pharm, Shanghai 201318, Peoples R China

年份：2017

卷号：97

起止页码：5

外文期刊名：CATALYSIS COMMUNICATIONS

收录：;EI(收录号：20171503565862);WOS:【SCI-EXPANDED(收录号:WOS:000401378200002)】；

基金：This work was supported by the National Natural Science Foundation of China (Grant No. 21406068/B060804), and the Fundamental Research Funds for the Central Universities (Grant No. 222201514324).

语种：英文

外文关键词：(R)-N-Boc-3-hydroxypiperidine; Carbonyl reductase; Asymmetric reduction; Cofactor-recycling system

摘要：The 3-hydroxypiperidine moiety is a privileged scaffold encountered in many bioactive compounds. An NADPH-dependent reductase (YGL039W) from Kluyveromyces marxianus ATCC 748 was isolated to show excellent catalytic activity in (R)-N-Boc-3-hydroxypiperidine [(R)-NBHP] production. Using a GDH-catalyzed cofactor recycling system to ensure a sufficient supply of NADPH, the effects of temperature, pH, metal ions, substrate concentration, biocatalyst dosage, and cofactors on the YGL039W-catalyzed bioreduction were investigated and optimized. Finally, an extremely high concentration of N-Boc-piperidin-3-one (NBPO, 400 g/L) could be completely reduced to (R)-NBHP (> 99% ee), with a total turnover number of 20,000. This process shows significant potential for the industrial production of (R)-NBHP.