文献类型：期刊文献

英文题名：A simple novel approach for real-time monitoring of sodium gluconate production by on-line physiological parameters in batch fermentation by Aspergillus niger

作者：Lu, Fei[1];Wang, Zejian[1];Zhao, Wei[2];Chu, Ju[1];Zhuang, Yingping[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shan Dong Fuyang Biol Technol Co Ltd, Shanghai, Peoples R China

年份：2016

卷号：202

起止页码：133

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20160301812248);WOS:【SCI-EXPANDED(收录号:WOS:000367673500018)】；

基金：This work was financially supported by grants from Major State Basic Research Development Program of China (973 Program) ( Nos. 2013CB733600 and 2012CB72100x), National High Technology Research and Development Program ( 2015AA021005) and the National Natural Science Foundation of China ( Grant No. 31200024). We also thank Shan Dong Fuyang Biological Technology Co., Ltd (DeZhou, China) for donating the industrial strain of A. niger.

语种：英文

外文关键词：Sodium gluconate; Aspergillus niger; On-line parameters; Real-time monitoring; Oxygen limited

摘要：In this paper, approach for real-time monitoring of sodium gluconate (SG) fermentation was established for the first time by the equations which can calculate real-time key-parameters by on-line physiological data. Based on this approach, limiting factors were found out in initial fermentation F1 and then step-wise agitation increase and improved medium recipe were proposed in fermentation F2 and F3, respectively. The highest average SG production rate (16.58 +/- 0.91 g L (1) h (1)) was achieved in fermentation F3, which was 104.2% and 48.0% higher than those in fermentation F1 and F2, respectively. Meanwhile, due to shorter fermentation period (decreased from 34 h to 18.7 h), lower biomass (about 1.5 g L (1)) and less by-product accumulation, the overall yield of 0.943 +/- 0.012 (mol mol (1)) in fermentation F3 increased more than 16.0% compared to fermentation F1. This approach had been successfully applied to industrial fermentation and greatly improved SG production. (C) 2015 Elsevier Ltd. All rights reserved.