文献类型：期刊文献

英文题名：Evaluation of absorbable hemostatic agents of polyelectrolyte complexes using carboxymethyl starch and chitosan oligosaccharide both in vitro and in vivo

作者：Chen, Xingtao[1];Yan, Yonggang[1];Li, Hong[1];Wang, Xuehong[2];Tang, Songchao[2];Li, Quan[3];Wei, Jie[2];Su, Jiacan[3]

机构：[1]Sichuan Univ, Coll Phys Sci & Technol, Chengdu 610064, Sichuan, Peoples R China;[2]East China Univ Sci & Technol, Minist Educ, Key Lab Ultrafine Mat, Shanghai 200237, Peoples R China;[3]Second Mil Med Univ, Changhai Hosp, Dept Orthopaed Trauma, Shanghai 200433, Peoples R China

年份：2018

卷号：6

期号：12

起止页码：3332

外文期刊名：BIOMATERIALS SCIENCE

收录：;EI(收录号：20184806150102);WOS:【SCI-EXPANDED(收录号:WOS:000450897100016)】；

基金：This work was supported by the National Natural Science Foundation of China (51772194, 51502340 and 81771990) and Key Medical Program of Science and Technology Development of Shanghai (17441900600 and 17441902000).

语种：英文

外文关键词：Starch - Azo dyes - Polyelectrolytes - Physicochemical properties - Chitosan - Biodegradation - Degradation - Water absorption - Escherichia coli - Particle size

摘要：Absorbable hemostatic agents with a high hemostatic efficacy play an important role in surgical and severely traumatic hemostasis. In the present study, by applying polyelectrolyte assembly, polyelectrolyte complexes (PECs), using carboxymethyl starch (CMS) and chitosan oligosaccharide (COS), with controllable physicochemical properties were prepared and optimized for hemorrhage control. Particle size, zeta potential, morphology and water absorption of the PECs with different CMS/COS ratios were systematically evaluated. The results of in vitro degradation in PBS suggested that CMS/COS PECs were degradable and their degradation rates, which decreased with the increase of the COS content, were suitable for absorbable hemostatic agents. The in vivo hemostatic efficacy of the PECs with 10 wt% COS content (PEC 10), which was evaluated in a rabbit hepatic hemorrhage model, was better than CMS but decreased with the increase of the COS content. The plasma coagulation evaluation revealed that the PECs could significantly activate and accelerate the coagulation cascade through both the intrinsic and extrinsic pathways but could not directly affect the common pathway. CMS/COS PECs also showed antimicrobial activity against S. aureus, which enhanced with the increase of the COS content, but failed against E. coli. Moreover, PEC 10 displayed excellent cytocompatibility with MC3T3-L1 and good tissue compatibility in a rabbit liver model. These findings not only suggest that CMS/COS PECs with a suitable COS content were promising absorbable hemostatic agents for internal use but they are also useful to understand the underlying principles for designing PEC based hemostatic agents.