文献类型：期刊文献

英文题名：Sensitive and Real-Time Assessment of DNA Methylation Degree by a Luminescent Lanthanide-Functionalized Mof Probe

作者：Chen, Guoli[1]; Hao, Ji-Na[1]; Li, Yongsheng[1,2]

机构：[1] Lab of Low-Dimensional Materials Chemistry, Key Laboratory for Ultrafine Materials of Ministry of Education, Frontier Science Center of the Materials Biology and Dynamic Chemistry, Shanghai Engineering Research Center of Hierarchical Nanomaterials, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai, 200237, China; [2] School of Chemistry and Chemical Engineering, State Key Laboratory Incubation Base for Green Processing of Chemical Engineering, Shihezi University, Shihezi, 832003, China

年份：2025

外文期刊名：SSRN

收录：EI(收录号：20250158854)

语种：英文

外文关键词：Alkylation - Body fluids - Europium alloys - Europium compounds - Metal-Organic Frameworks

摘要：5-Methylcytosine (5-mC), as one of the primary forms of DNA methylation, plays essential roles in a variety of biological activities. Its rapid and precise detection is therefore paramount for abnormal DNA methylation assessment and disease diagnosis, but still remains challenging. Herein, a highly luminescent and stable lanthanide-based metal-organic framework (MOF) probe, namely Eu-ZrMOF, is firstly designed for qualitative and quantitative analysis of 5-mC, wherein the organic building units of 1,3,5-benzenetricarboxylic acid (BTC) serve as both the energy donors and recognition molecules, and the modified Eu3+ ions function as intense signal reporters. Significantly, the luminescence of the fabricated Eu-ZrMOF probe can be remarkably quenched by 5-mC via its inner filter effect on the BTC absorption, achieving high response to 5-mC in a very short time (20 s). Moreover, such an efficient, instantaneous response probe presents excellent specificity towards 5-mC among various coexisting species in urine, and high sensitivity, thereby enabling it to be applied in accurately quantifying 5-mC content in real urine samples. This study demonstrates a simple, real-time and effective approach for sensitive identification of urinary 5-mC, prefiguring its great potential for the assessment of DNA methylation for the clinical diagnosis and prognosis of genetic diseases and tumors. ? 2025, The Authors. All rights reserved.