文献类型：期刊文献

英文题名：Multivalued Logic Assay of the Disease Marker of α-Ketoglutaric Acid by a Luminescent MOF-Based Biosensor

作者：Dai, Xu[1];Hao, Ji-Na[1];Gu, Jinlou[1];Li, Yongsheng[1]

机构：[1]East China Univ Sci & Technol, Frontier Sci Ctr Mat Biol & Dynam Chem, Sch Mat Sci & Engn,Shanghai Engn Res Ctr Hierarch, Lab Low Dimens Mat Chem,Key Lab Ultrafine Mat,Min, Shanghai 200237, Peoples R China

年份：2020

卷号：3

期号：6

起止页码：3792

外文期刊名：ACS APPLIED BIO MATERIALS

收录：EI(收录号：20202708894124);WOS:【ESCI(收录号:WOS:000604590400035)】；

基金：This work was financially supported by the National Key Research and Development Program of China (grant no. 2018YFC1105702), the National Natural Science Foundation of China (grant nos. 51621002, 21805087 and 51972112), Shanghai Sailing Program (18YF1405900), China Postdoctoral Science Foundation (2017M621383), Program of Shanghai Academic/Technology Research Leader (18XD1401400), Basic Research Program of Shanghai (17JC1404702), Leading Talents in Shanghai in 2018, 111 project (B14018), and the Fundamental Research Funds for Central Universities.

语种：英文

外文关键词：luminescent MOF; biosensor; multivalued logic assay; disease marker; alpha-ketoglutaric acid

摘要：alpha-Ketoglutaric acid (alpha-KA) is an important endogenous metabolite in the Krebs cycle and considered a critical marker for various diseases. Several approaches for identifying alpha-KA have been reported. However, most of them are limited to single-signal transduction modes in one assay and working in the suspension state, which easily cause quantification errors and lead to false-positive detection results. Herein, a multivalue-responsive and semi-solid EuMOF-gel (EuMOG) logic biosensor is first designed and fabricated, which can realize the specific, rapid, and easy-to-differentiate naked-eye detection of the disease-associated marker of alpha-KA in serum through implementing one-to-two logic operation with three multicolor gates. The implementation of one-to-two logic operation can be rationally achieved by assembling 2,6-naphthalenedicarboxylic acid as both the energy donor and blue-colored output 1, photoactive Eu3+ ions as the red-emissive output 2, and alpha-KA stimuli as an input and energy blocker between the dual spectrum-resolved outputs into one unit. With the specific input of alpha-KA among various analytes, the binary code of the logic decoder switches from (0,1) to (1,0) with an obvious color change from red to cyan. Moreover, by integrating the output chromaticity into the logical operations, the concentration levels of alpha-KA could be easily evaluated with the intelligent EuMOG detector, which has three multicolor gates in parallel encoding low (0,1), normal (1,1), and danger (1,0) statuses. Such multivalued logic biosensor provides a facile strategy to improve the analysis reliability of disease-associated biomarkers.