文献类型：期刊文献

英文题名：Highly efficient enrichment of phosphopeptides from HeLa cells using hollow magnetic macro/mesoporous TiO2 nanoparticles

作者：Hong, Yayun[1];Zhan, Qiliang[1];Pu, Chenlu[1];Sheng, Qianying[1];Zhao, Hongli[1];Lan, Minbo[1,2]

机构：[1]East China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2018

卷号：187

起止页码：223

外文期刊名：TALANTA

收录：;EI(收录号：20182005205138);WOS:【SCI-EXPANDED(收录号:WOS:000445302600030)】；

基金：This research was financially supported by the Science and Technology Commission of Shanghai Municipality (STCSM, No. 15441904100), the Fundamental Research Funds for the Central Universities (No. 222201817022), the Natural Science Foundation of Shanghai (No. 16ZR1407600) and Shanghai Sailing Program (No. 16YF1402400).

语种：英文

外文关键词：Hollow magnetic macro/mesoporous TiO2; Phosphopeptide enrichment; High selectivity; High-efficiency; HeLa cell extracts

摘要：In this work, hollow magnetic macro/mesoporous TiO2 nanoparticles (denoted as Fe3O4@H-fTiO(2)) were synthesized by a facile "hydrothermal etching assisted crystallization" route to improve the phosphopeptide enrichment efficiency. The porous nanostructure of TiO2 shell and large hollow space endowed the Fe3O4@H-fTiO(2) with a high surface area (144.71 m(2) g(-1)) and a large pore volume (0.52 cm(3) g (-1)), which could provide more affinity sites for phosphopeptide enrichment. Besides, the large pore size of TiO2 nanosheets and large hollow space could effectively prevent the "shadow effect", thereby facilitating the diffusion and release of phospho-peptides. Compared with the hollow magnetic mesoporous TiO2 with small and deep pores (denoted as Fe3O4@H-mTiO(2)) and solid magnetic macro/mesoporous TiO2, the Fe3O4@H-fTiO(2) nanoparticles showed a better selectivity (molar ratio of alpha-casein/BSA up to 1:10000) and a higher sensitivity (0.2 fmol/mu L alpha-casein) for phosphopeptide enrichment. Furthermore, 1485 unique phosphopeptides derived from 660 phosphoproteins were identified from HeLa cell extracts after enrichment with Fe2O4@H-fTiO(2) nanoparticles, further demonstrating that the Fe3O4@H-fTiO(2) nanoparticles had a high-efficiency performance for phosphopeptide enrichment. Taken together, the Fe3O4@H-fTiO(2) nanoparticles will have unique advantages in phosphoproteomics analysis.