文献类型：期刊文献

英文题名：Oxygen-enriched fermentation of sodium gluconate by Aspergillus niger and its impact on intracellular metabolic flux distributions

作者：Shen, Yuting[1];Tian, Xiwei[1];Zhao, Wei[2];Hang, Haifeng[1];Chu, Ju[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, POB 329,130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Shan Dong Fuyang Biol Technol Co Ltd, Dezhou, Peoples R China

年份：2018

卷号：41

期号：1

起止页码：77

外文期刊名：BIOPROCESS AND BIOSYSTEMS ENGINEERING

收录：;EI(收录号：20174104245461);WOS:【SCI-EXPANDED(收录号:WOS:000419458800007)】；

基金：This work was funded by grants from Major State Basic Research Development Program of China (973 Program) (Nos. 2013CB733600 and 2012CB72100x), National High Technology Research and Development Program (2015AA021005) and the National Natural Science Foundation of China (Grant no. 31200024). We also appreciated Shan Dong Fuyang Biological Technology Co., Ltd (Dezhou, China) for donating the industrial strain of A. niger.

语种：英文

外文关键词：Gluconic acid; Aspergillus niger; Oxygen; Metabolic flux distribution

摘要：Different concentrations of oxygen-enriched air were utilized for sodium gluconate (SG) fermentation by Aspergillus niger. The fermentation time shortened from 20 to 15.5 h due to the increase of volumetric oxygen transfer coefficient (K(L)a) and the formation of more dispersed mycelia when inlet oxygen concentration ascended from 21 to 32%. According to metabolic flux analysis, during the growth phase, extracellular glucose for SG synthesis accounted for 79.0 and 85.3% with air and oxygen-enriched air (25%), respectively, whereas the proportions were 89.4 and 93.0% in the stationary phase. Intracellular glucose consumption decreased in oxygen-enriched fermentation, as cell respiration was more high-efficiently performed. Metabolic profiling indicated that most intermediates in TCA cycle and EMP pathway had smaller pool sizes in oxygen-enriched fermentations. Moreover, the main by-product of citric acid dramatically decreased from 1.36 to 0.34 g L-1 in oxygen-enriched fermentation. And the sodium gluconate yield increased from 0.856 to 0.903 mol mol(-1).