文献类型：期刊文献

英文题名：Fabrication of carbon nanotube-polylactic acid composite electrode by melt compounding for capillary electrophoretic determination of tectoridin and irigenin in Belamcandae Rhizoma

作者：Mao, Hua[1,2];Ye, Xinming[3];Chen, Weilong[4];Geng, Wenye[1,2];Chen, Gang[1,2]

机构：[1]Fudan Univ, Affiliated Pudong Med Ctr, 826 Zhangheng Rd, Shanghai 201203, Peoples R China;[2]Fudan Univ, Sch Pharm, Pharmaceut Anal Dept, 826 Zhangheng Rd, Shanghai 201203, Peoples R China;[3]East China Univ Sci & Technol, Sch Sports Sci & Engn, Shanghai 200237, Peoples R China;[4]Phenom Sci Instrument Shanghai Co Ltd, Shanghai 201203, Peoples R China

年份：2019

卷号：175

外文期刊名：JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000485206900018)】；

基金：This work was financially supported by Shanghai Municipal Science and Technology Commission (16391903600 and 16DZ0501200) and the Natural Science Foundation of China (21375023).

语种：英文

外文关键词：Amperometric detection; Capillary electrophoresis; Carbon nanotubes; Detection electrode; Melt compounding; Polylactic acid

摘要：y A sensitive detection electrode based on the composite of carbon nanotubes (CNTs) and polylactic acid (PLA) was fabricated for measuring the bioactive constituents in Belamcandae Rhizoma by capillary electrophoresis (CE). The composite was facilely fabricated by packing a blend of CNTs and melted PLA into a fused silica capillary under heat. The prepared CNT-PLA composite was characterized by scanning electron microscopy, Raman spectroscopy, Fourier transform infrared spectroscopy and cyclic voltammetry. The results indicated that PLA bound electrically conductive CNTs to form a well-dispersed composite network for electrochemical sensing. The electrode was employed to the off-line detection of tectoridin and irigenin in Belamcandae Rhizoma to demonstrate its performance in capillary electrophoresis. At a separation voltage of 12 kV, the two isoflavones were well separated and detected within 8 min in a 40-cm fused silica capillary in a borate buffer at pH 9.8. The detection limits for tectoridin and irigenin were measured to be 0.24 and 0.21 mu M, respectively. (C) 2019 Elsevier B.V. All rights reserved.