文献类型：期刊文献

英文题名：Diversity and degradation mechanism of an anaerobic bacterial community treating phenolic wastewater with sulfate as an electron acceptor

作者：Guo, X. J.[1];Lu, Z. Y.[1];Wang, P.[3];Li, H.[1];Huang, Z. Z.[1,2];Lin, K. F.[1];Liu, Y. D.[1]

机构：[1]E China Univ Sci & Technol, Sch Resource & Environm Engn, State Environm Protect Key Lab Environm Risk Asse, State Key Lab Biol Reactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Univ Engn Sci, Sch Chem Engn, Shanghai 201620, Peoples R China;[3]E China Univ Sci & Technol, Sch Bioengn, Shanghai 200237, Peoples R China

年份：2015

卷号：22

期号：20

起止页码：16121

外文期刊名：ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH

收录：;EI(收录号：20242616498991);WOS:【SCI-EXPANDED(收录号:WOS:000363966900085)】；

基金：Supported jointly by the National Natural Science Foundation of China (51378208, 41273109), Specialized Research Fund for the Doctoral Program of Higher Education (20110074130002), Program for New Century Excellent Talents in University (NCET-13-0797), Innovation Program of Shanghai Municipal Education Commission (14ZZ059), and Fundamental Research Funds for the Central Universities (222201313008). We would like to thank the anonymous referees for their helpful comments regarding this paper.

语种：英文

外文关键词：Phenolic wastewater; High sulfate concentration; Anaerobic treatment; Bacterial community; Degradation mechanism; Real-time PCR

摘要：Petrochemical wastewater often contains high concentrations of phenol and sulfate that must be properly treated to meet discharge standards. This study acclimated anaerobic-activated sludge to treat saline phenolic wastewater with sulfate reduction and clarified the diversity and degradation mechanism of the microbial community. The active sludge in an upflow anaerobic sludge blanket (UASB) reactor could remove 90 % of phenol and maintain the effluent concentration of SO42- below 400 mg/L. Cloning and sequencing showed that Clostridium spp. and Desulfotomaculum spp. were major phenol-degrading bacteria. Phenol was probably degraded through the carboxylation pathway and sulfate reduction catalyzed by adenosine-5'-phosphosulfate (APS) reductase and dissimilatory sulfite reductase (DSR). A real-time polymerase chain reaction (RT-PCR) showed that as phenol concentration increased, the quantities of 16S rRNA gene, dsrB, and mcrA in the sludge all decreased. The relative abundance of dsrB dropped to 12.46 %, while that of mcrA increased to 56.18 %. The change in the electron flow ratio suggested that the chemical oxygen demand (COD) was removed mainly by sulfate-reducing bacteria under a phenol concentration of 420 mg/L, whereas it was removed mainly by methanogens above 630 mg/L.