文献类型：期刊文献

英文题名：Phosphate limitation increases coenzyme Q10 production in industrial Rhodobacter sphaeroides HY01

作者：Zhang, Lu[1,2];Liu, Leshi[1,2];Wang, Ke-Feng[1,2];Xu, Lan[3,4];Zhou, Liming[1,2];Wang, Weishan[3,4];Li, Chuan[1,2];Xu, Zheng[3,4,8];Shi, Tong[1,2];Chen, Haihong[1,2];Li, Yuanhang[1,2];Xu, Hui[5];Yang, XiuLiang[6];Zhu, Zhichun[7];Chen, Biqin[7];Li, Dan[7];Zhan, Guanghuang[7];Zhang, Si-Liang[1,2];Zhang, Li-Xin[1,2];Tan, Gao-Yi[1,2]

机构：[1]ECUST, SKLBE, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]ECUST, Sch Biotechnol, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]Chinese Acad Sci, State Key Lab Microbial Resources, Inst Microbiol, 1 Beichen West Rd, Beijing 100101, Peoples R China;[4]Chinese Acad Sci, CAS Key Lab Pathogen Microbiol & Immunol, Inst Microbiol, 1 Beichen West Rd, Beijing 100101, Peoples R China;[5]Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Liaoning, Peoples R China;[6]Shandong Jincheng Biopharmaceut Co Ltd, 117 Qixing River Rd, Zibo 255130, Peoples R China;[7]Inner Mongolia Kingdomway Pharmaceut Co Ltd, Tuoketuo Power Ind Pk, Hohhot 010206, Peoples R China;[8]Beijing Inst Educ, Dept Biol, Beijing 100044, Peoples R China

年份：2019

卷号：4

期号：4

起止页码：212

外文期刊名：SYNTHETIC AND SYSTEMS BIOTECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000503371600006)】；

基金：The authors appreciate Dr. Jin Miao for the help to construct engineered strains in Table 1. The author also appreciates Prof. Hongwei Yu for providing plasmid materials. This work was supported by the National Natural Science Foundation of China [31870040, 31430002, 31720103901], the 111 Project of China [B18022], the Fundamental Research Funds for the Central Universities [22221818014], the Natural Science Foundation of Shandong Province [ZR2017ZB0206], and the Shandong Taishan Scholar Award to Lixin Zhang.

语种：英文

外文关键词：R sphaeroides; CoQ(10); Phosphate limitation; Overproduction; Scale-up; Transcriptome

摘要：Coenzyme Q(10) (CoQ(10)) is an important component of the respiratory chain in humans and some bacteria. As a high-value-added nutraceutical antioxidant, CoQ(10) has excellent capacity to prevent cardiovascular disease. The content of CoQ(10) in the industrial Rhodobacter sphaeroides HY01 is hundreds of folds higher than normal physiological levels. In this study, we found that overexpression or optimization of the synthetic pathway failed CoQ(10) overproduction in the HY01 strain. Moreover, under phosphate-limited conditions (decreased phosphate or in the absence of inorganic phosphate addition), CoQ(10) production increased significantly by 12% to220 mg/L, biomass decreased by 12%, and the CoQ(10) productivity of unit cells increased by 27%. In subsequent fed-batch fermentation, CoQ(10) production reached 272 mg/L in the shake-flask fermentation and 1.95 g/L in a 100-L bioreactor under phosphate limitation. Furthermore, to understand the mechanism associated with CoQ(10) overproduction under phosphate-limited conditions, the comparatve transcriptome analysis was performed. These results indicated that phosphate limitation combined with glucose fed-batch fermentation represented an effective strategy for CoQ(10) production in the HY01. Phosphate limitation induced a pleiotropic effect on cell metabolism, and that improved CoQ(10) biosynthesis efficiency was possibly related to the disturbance of energy metabolism and redox potential.