文献类型：期刊文献

中文题名：生防菌Pseudomonas fluorescens 2P24对甜瓜根围土壤微生物的影响

英文题名：Effects of the Biocontrol Agent Pseudomonas fluorescens 2P24 on Microbial Community Diversity in the Melon Rhizosphere

作者：朱伟杰[1];王楠[1];郁雪平[1];王伟[1]

机构：[1]华东理工大学/生物反应器工程国家重点实验室,上海200237

年份：2010

卷号：43

期号：7

起止页码：1389

中文期刊名：中国农业科学

外文期刊名：Scientia Agricultura Sinica

收录：CSTPCD;;Scopus;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金：国家自然科学基金项目(30871664);国家"863"计划重大项目(2006AA10A211)

语种：中文

中文关键词：生防荧光假单胞菌2P24;根围土壤微生物;T-RFLP

外文关键词：biocontrol agent Pseudomonasfluorescens 2P24; rhizosphere microorganism; T-RFLP

摘要：【目的】探讨生防假单胞菌2P24对根围土壤微生物的影响,为生防菌的安全应用提供基础。【方法】利用平板培养计数与末端标记限制性片段长度多态性分析(terminal restriction fragment length polymorphism,T-RFLP)相结合的方法研究施用生防假单胞菌2P24后不同生育期甜瓜根围土壤微生物多样性的变化。【结果】在甜瓜定植后,生防菌2P24对土壤中细菌和真菌均有较强的抑制作用,对放线菌却具有促进作用。在收获期,2P24对土壤中细菌和放线菌的影响逐渐减弱,而对真菌表现了一定的促进作用。获得了41个细菌菌群的TRF片段,其中,2P24对优势菌群TRF213、TRF240、TRF513无明显影响,而对TRF61、TRF348、TRF365等菌群影响显著,土壤微生物Shannon-Wiener多样性指数有所提高。【结论】将传统培养法和T-RFLP分析技术相结合,是分析土壤微生物种群变化较为理想的方法。生防菌2P24对甜瓜根围土壤微生物的生态系统影响不显著。
[Objective] The objective of this study was to investigate the influence of biocontrol agent Pseudomonas fluorescens 2P24 on microecosystem in the rhizosphere of melon, and to provide biosafety evaluation for the application of the bioagent. [Method] The influence of biocontrol agent 2P24 on microecosystem in melon rhizosphere at different growing stages was investigated by using plate culture and terminal restriction fragment length polymorphism （T-RFLP） analysis. [Result] After transplantion, the rhizosphere biomass of bacteria and fungi in the strain 2P24-treated melon seedlings was decreased, while the population of actinomycetes was increased. In the harvest period, the influence of strain 2P24 on bacteria and actinomycetes was not significant, but the fungal population was promoted obviously. Totally 41 TRFs were obtained by T-RFLP analysis, among which the dominant floras of TRF213, TRF240 and TRF513 were not obviously affected, while the floras of TRF61, TRF348 and TRF365 were influenced significantly,wich was also validated by the increased biodiversity indices（Shannon-Wiener）. [Conclusion] The abundance bioinformation on the influence of strain 2P24 on microbial diversity was obtained by the methods combined with plate cultivation and T-RFLP analysis, and the results showed that the biocontrol agent 2P24 did not significantly affect the diversity and dynamics of microbial communities in melon rhizosphere.