文献类型：期刊文献

中文题名：芽孢杆菌腺苷磷酸化酶在大肠杆菌中的表达及酶学特性

英文题名：Expression of deoD Gene Coding for Adenosine Phosphorylase from Bacillus sp. in E. coli and Enzymatic Properties

作者：高淑红[1];全敏[1];胡慧莉[1];陈长华[1];张嗣良[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室国家生化工程技术研究中心,上海200237

年份：2007

卷号：38

期号：9

起止页码：629

中文期刊名：中国医药工业杂志

外文期刊名：Chinese Journal of Pharmaceuticals

收录：CSTPCD;;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：腺苷磷酸化酶;deoD;蛋白表达;底物特异性

外文关键词：adenosine phosphorylase; deoD; protein expression; substrate specificity

摘要：对芽孢杆菌的腺苷磷酸化酶基因deoD在大肠杆菌BL21(DE3)中的表达条件进行优化。确定在菌体培养的对数生长中期进行诱导,异丙基-β-D-硫代半乳糖苷(IPTG)诱导浓度为0.05mmol/L,诱导温度为37℃。腺苷磷酸化酶的最适pH为7.0,反应温度为50℃,其底物范围广,对腺苷、肌苷均有催化活性。
The expression of deoD gene from Bacillus sp. in E. coli BL21 （DE3）was studied. To achieve the high level of expression, IPTG was added in the middle exponential growth period up to 0.05mmol/L at 37℃. The properties of adenosine phosphorylase were investigated. The optimum pH value and temperature for this enzyme were 7.0 and 50℃. It was showed that this enzyme possessed a broad specificity to the substrates including both adenosine and inosine.