文献类型：期刊文献

英文题名：Rational design of a turn-on fluorescent sensor for α-ketoglutaric acid in a microfluidic chip

作者：Jin, Pengwei;Jiao, Changhong;Guo, Zhiqian[1];He, Ye;Zhu, Shiqin;Tian, He;Zhu, Weihong

机构：[1]E China Univ Sci & Technol, Key Lab Adv Mat, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China; E China Univ Sci & Technol, Inst Fine Chem, Shanghai 200237, Peoples R China

年份：2014

卷号：5

期号：10

起止页码：4012

外文期刊名：CHEMICAL SCIENCE

收录：;EI(收录号：20143618131464);WOS:【SCI-EXPANDED(收录号:WOS:000341195100041)】；

基金：This work was supported by the National 973 Program (no. 2013CB733700), the NSFC for Distinguished Young Scholars (Grant no. 21325625), NSFC/China, the Oriental Scholarship, Shanghai Pujiang Program (13PJD010), the Fok Ying Tong Education Foundation (142014), the Fundamental Research Funds for the Central Universities (WK1013002, 222201313010), and SRFDP 20120074110002.

语种：英文

外文关键词：Fluorescence - Chromatographic analysis - Body fluids - Microfluidics

摘要：Detection of biomarkers via optical microfluidic chips is in great demand for high contrast biosensing and bioimaging. In distinct contrast with traditional chromatographic methods, which require tedious pretreatment and are not directly applicable in blood serum, a "turn-on" fluorescent sensor for the cancer cell damaging agent alpha-ketoglutaric acid (alpha-KA) has been established. A hydrazino group is introduced into the naphthalimide moiety as the reaction trigger for the specific fluorescence turn-on response. Under the rational design, probe 3 can successfully detect alpha-KA in a purely aqueous system, along with approximately 7-fold fluorescence enhancement and a rapid response with the aid of micelles. The sensor exhibits good selectivity among 20 common amino acids, in particular showing little interference with various dicarbonyl derivatives and reactive oxygen species. Finally, the detection of alpha-KA in human serum is demonstrated in a microfluidic chip, indicative of a potential platform for high-throughput screening and monitoring of kinetics, especially in biological fields.