文献类型：期刊文献

英文题名：Redox-Mediated Indirect Fluorescence Immunoassay for the Detection of Disease Biomarkers Using Dopamine-Functionalized Quantum Dots

作者：Zhang, Wen-Hui;Ma, Wei[1];Long, Yi-Tao

机构：[1]E China Univ Sci & Technol, Key Lab Adv Mat, 130 Meilong Rd, Shanghai 200237, Peoples R China; E China Univ Sci & Technol, Dept Chem, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2016

卷号：88

期号：10

起止页码：5131

外文期刊名：ANALYTICAL CHEMISTRY

收录：;EI(收录号：20162202452956);WOS:【SCI-EXPANDED(收录号:WOS:000376223500018)】；

基金：This research was supported by the 973 Program (2013CB733700, 2014CB748500), National Natural Science Foundation of China (21125522, 21305045), and Fundamental Research Funds for the Central Universities (222201313004).

语种：英文

外文关键词：II-VI semiconductors - Cadmium compounds - Immunology - Chemical detection - Fluorescence quenching - Neurophysiology - Semiconductor quantum dots - Biomarkers - Nanocrystals

摘要：Here, we report a redox-mediated indirect fluorescence immunoassay (RMFIA) for the detection of the disease biomarker alpha-fetoprotein (AFP) using dopamine (DA)-functionalized CdSe/ZnS quantum dots (QDs). In this immunoassay, tyrosinase was conjugated with the detection antibody and acted as a bridge connecting the fluorescence signals of the QDs with the concentration of the disease biomarkers. The tyrosinase label used for RMFIA catalyzed the enzymatic oxidation of DAs on the surface of functionalized QDs and caused fluorescence quenching in the presence of the analyte. Using this technique, we obtained a limit of detection as low as 10 pM for AFP. This assay's potential for clinical analysis was demonstrated by detecting the real sera of patients with hepatocellular carcinoma (HCC). This study makes the first use of RMFIA for the rapid detection of AFP, opening up a new pathway for the detection of disease biomarkers.