文献类型：期刊文献

英文题名：Efficacy of chitosan oligosaccharide as aquatic adjuvant administrated with a formalin-inactivated Vibrio anguillarum vaccine

作者：Liu, Xiaohong[1,2];Zhang, Hua[2];Gao, Yuan[1];Zhang, Yang[2];Wu, Haizhen[1];Zhang, Yuanxing[1,2,3]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Engn Res Ctr Maricultured Anim Vaccines, Shanghai, Peoples R China;[3]Shanghai Collaborat Innovat Ctr Biomfg Technol, Shanghai, Peoples R China

年份：2015

卷号：47

期号：2

起止页码：855

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000366874500026)】；

基金：This work was financially supported by National Programs for High Technology Research and Development Program of China (2014AA093604), National Science-technology Support Plan Projects (2012BAD17B02), and Shanghai Rising-Star Program (13QA1401000).

语种：英文

外文关键词：Water-soluble adjuvant; Chitosan oligosaccharide; Formalin-inactivated vaccine; Vibrio anguillarum

摘要：Vaccine is one of the efficient candidates to prevent fish disease through activating host immune response in aquaculture. Actually, several vaccines are often administered with adjuvants to increase immunostimulation, especially for some water-based formalin-killed vaccines. However, side effects are inevitable after vaccination of some adjuvants. Therefore, exploration for effective and harmless aquatic adjuvants is urgently needed. In this study, immunoprotection of a formalin-inactivated Vibrio anguillarum vaccine applied with chitosan oligosaccharide (COS) was analyzed. High levels of protection were achieved in zebrafish and turbot vaccinated with inactivated vaccine and COS (RPS of 89.0 +/- 4.5% and 80.0 +/- 6.9%) compared with fish vaccinated with inactivated vaccine alone (RFS of 47.8 +/- 6.6% and 64.7 +/- 5.8%) at 4 week post vaccination. Moreover, high antibody reaction and cross-protection against Vibrio alginolyticus and Vibrio harveyi were observed of turbot vaccinated with inactivated vaccine and COS. In conclusion, COS can enhance immunoprotection of a formalin-inactivated V. anguillarum vaccine, significantly activate humoral immune response of host, and be benefit for inhibition against pathogens. Therefore, COS would be a potential adjuvant for aquatic vaccine design in the future. (C) 2015 Elsevier Ltd. All rights reserved.