文献类型：期刊文献

英文题名：Enhanced anticancer efficacy and tumor targeting through folate-PEG modified nanoliposome loaded with 5-fluorouracil

作者：Le, Van Minh[1,2]; Nho, Trung Duc Tran[1]; Ly, Hai Trieu[2]; Vo, Thanh Sang[1]; Nguyen, Hoang Dung[1,5]; Phung, Thi Thu Huong[1]; Zou, Aihua[3]; Liu, Jianwen[4]

机构：[1] NTT Hi-Tech Institute, Nguyen Tat Thanh University, 300A Nguyen Tat Thanh road, District 4, Ho Chi Minh City, Viet Nam; [2] Research Center of Ginseng and Medicinal Materials [CGMM], National Institute of Medicinal Materials, Ho Chi Minh City, 70000, Viet Nam; [3] Institute of Applied Chemistry, East China University of Science and Technology, 130 Meilong Road, Shanghai, China; [4] Department of Molecular and Cellular Pharmacology, Biomedical Nanotechnology Center, School of Pharmacy, East China University of Science and Technology, 130 Meilong Road, Shanghai, China; [5] Institute of Tropical Biology, Vietnam Academy of Science and Technology, Viet Nam

年份：2017

卷号：8

期号：1

外文期刊名：Advances in Natural Sciences: Nanoscience and Nanotechnology

收录：EI(收录号：20203809196516)

语种：英文

外文关键词：Controlled drug delivery - Organic acids - Liposomes - Diseases - Cell death - Tumors

摘要：Cancer targeted therapies have attracted considerable attention over the past year. Recently, 5-fluouracil (5-FU), which has high toxicity to normal cells and short half-life associated with rapid metabolism, is one of the most commonly used therapies in the treatment of cancer. In this study the folic acid-conjugated pegylated nanoliposomes were synthesized and then loaded into them with 5-FU to improve the anti-tumor efficacy. The average size of liposomes (LPs) was about 52.7 nm which was identified by TEM. In the liposome uptake studies, the level uptake of folate-conjugated liposomes has increased compared to non-conjugated LPs according to LPs concentration, incubation time and presence of concentration of free folic acid (FA). The MTT assay and apoptotic test were carried out in HCT116 and MCF-7 cells for 24 or 48 h. The results revealed that the folate-PEG modified 5-Fu loaded nanoliposomes had strong cytotoxicity to cancer cell compared to pure 5-FU or PEG modified 5-FU loaded liposomes in a concentration- and time-dependent manner, and mainly enhanced the cancer cell death through folate-mediated endocytosis. Hence, the folate-PEG modified nanoliposome is a potential targeted drug-delivery system for the treatment of FR-positive cancers. ? 2017 Vietnam Academy of Science & Technology.