文献类型：期刊文献

英文题名：Whole cell microbial transformation in cloud point system

作者：Wang, Zhilong[1]; Xu, Jian-He[2]; Chen, Daijie[3]

机构：[1] School of Pharmacy, Shanghai Jiao Tong University, 200240 Shanghai, China; [2] Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 200237 Shanghai, China; [3] Shanghai Institutes of Pharmacy Industry, 200040 Shanghai, China

年份：2008

卷号：35

期号：7

起止页码：645

外文期刊名：Journal of Industrial Microbiology and Biotechnology

收录：EI(收录号：20242616502261)

语种：英文

外文关键词：Biocompatibility - Nonionic surfactants - Phase separation - Solvent extraction

摘要：Cloud point system, consisting of nonionic surfactant in an aqueous solution, has been developed as a novel medium for whole cell microbial transformation. The basic properties of cloud point system including phase separation and solubilization are introduced. The application of cloud point system for extractive microbial transformation is different from that of water-organic solvent two-phase partitioning system or aqueous two-phase system are discussed, which mainly focus on the biocompatibility of microorganism in a cloud point system and a downstream process of microbial transformation in cloud point system with oil-water-surfactant microemulsion liquid-liquid extraction for surfactant recovery and product separation. Finally, examples of whole cell microbial transformation in cloud point systems, especially in situ extraction of moderate polar substrate/product, are also presented. ? 2008 Society for Industrial Microbiology.