文献类型：期刊文献

中文题名：二维离子色谱法同时测定4种无机阴离子和葡萄糖酸根离子

英文题名：Determination of inorganic anions and gluconate by two-dimensional ion chromatography

作者：陈爱连[1];丁卉[1];方琳美[1];施超欧[1]

机构：[1]华东理工大学分析测试中心,上海200237

年份：2015

卷号：33

期号：12

起止页码：1333

中文期刊名：色谱

外文期刊名：Chinese Journal of Chromatography

收录：CSTPCD;;Scopus;WOS:【ESCI(收录号:WOS:000435257900015)】；北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；PubMed;

语种：中文

中文关键词：二维离子色谱;电导检测;脉冲安培检测;无机阴离子;葡萄糖酸根

外文关键词：two-dimensional ion chromatography; conductivity; pulsed amperometric; inorganic anion; gluconate;

摘要：建立了一种新的二维离子色谱分析模式,应用阀切换技术并联抑制电导和脉冲安培双检测体系,同时测定Cl^-、NO_2^-、SO_4^(2-)、NO_3^-和葡萄糖酸根离子。第一维色谱采用Ionpac AG18+Ionpac AS18阴离子分析柱,分别以5和20 mmol/L的NaOH溶液等度淋洗,流速为1.0 mL/min,进样量为25μL,抑制电导检测Cl^-、NO_2^-、SO_4^(2-)和NO_3^-。第二维色谱采用CarboPac PA1+CarboPac PA20两保护柱串联,以90 mmol/L NaOH溶液、0.8 mL/min的流速洗脱,由AG15柱分离富集葡萄糖酸根,脉冲安培检测器检测。结果表明:无机阴离子在0.1~5.0 mg/L、葡萄糖酸根在0.085 6~4.282 5 mg/L范围内有良好的线性关系,RSD在1.05%~1.94%之间,相关系数(R2)在0.994 5以上;无机阴离子的方法检出限为0.615~2.17μg/L,葡萄糖酸根的方法检出限为24.24μg/L;回收率在90.3%~102.8%之间。该方法并联两种检测模式,有良好的准确度和精密度,适用于复杂样品的分离分析。
A new two-dimensional ion chromatography method was developed to parallelly analyze two different types of samples with the application of valve switching technology-suppressed conductivity and pulsed amperometric analysis system,for concurrent determination of chloride,nitrite,sulfate,nitrate four inorganic anions and gluconate. The first dimensional chromatography was using Ionpac AG18＋Ionpac AS18 anion analysis columns with a suppressed conductivity detector for the separation and detection of Cl^-, NO2^-, SO4^2-and NO3^-.Respectively,the elution was 5 and 20 mmol / L NaOH at an isocratic flow rate of 1. 0 mL / min and sample injection volume of 25 μL. The second dimensional chromatography was utilizing two guard columns,CarboPac PA1 and CarboPac PA20,with 90 mmol / L NaOH solution for the isocratic eluent of 0. 8 mL / min. Gluconate was enriched by an AG15 column and switched into the pulsed amperometric detector. The results showed that：each inorganic anion in 0. 1- 5. 0mg / L and gluconate in 0. 085 6-4. 282 5 mg / L had a good linear relationship（R2≥0. 994 5）. The RSDs of the peak areas were between 1. 05%- 1. 94%. The limits of detection were 0. 61- 2. 17μg / L for the anions and 24. 24 μg / L for the gluconate. The recoveries were between 90. 3%-102. 8%. The two detection modes parallelly have good separation efficiency,detection accuracy and the precision of the separation and are suitable for the analysis of complex samples.