文献类型：期刊文献

英文题名：Optical Control of Invertebrate nAChR and Behaviors with Dithienylethene-Imidacloprid

作者：Xu, Qi[1];Zhang, Chao[1];Xu, Zhiping[1];Wang, Long[1];Liu, Zewen[2];Li, Zhong[1];Shao, Xusheng[1,3,4]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China;[2]Nanjing Agr Univ, Coll Plant Protect, Key Lab Integrated Management Crop Dis & Pests, Minist Educ, Nanjing 210095, Peoples R China;[3]East China Univ Sci & Technol, Sch Pharm, State Key Lab Bioreactor Engn, Shanghai Frontier Sci Res Base Optogenet Tech Cell, Shanghai 200237, Peoples R China;[4]East China Univ Sci & Technol, Engn Res Ctr Pharmaceut Proc Chem, Sch Pharm, Minist Educ, Shanghai 200237, Peoples R China

年份：2023

卷号：71

期号：29

起止页码：11048

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;EI(收录号：20233014453259);WOS:【SCI-EXPANDED(收录号:WOS:001024802900001)】；

语种：英文

外文关键词：photopharmacology; nAChR; photoswitchable; dithienylethene; imidacloprid

摘要：Photopharmacologyinvolving azobenzene or dithienylethene has changedestablished methods of studying receptor functions, allowing for increasingthe spatiotemporal resolution. There are no photopharmacological toolsavailable for the invertebrate nicotinic acetylcholine receptor (nAChR).Here, we report a photochromic ligand, dithienylethene-imidacloprid(DitIMI), using a dithienylethene photoswitch embedded in the nAChRagonist imidacloprid. It was found that DitIMI displayed good photochromismand fluorescence switching behaviors upon irradiation with UV/vislight in aqueous solution. We demonstrated that open-DitIMI has low spontaneous in vitro and in vivo activity but canbe photoisomerized to a highly active closed-form.Surprisingly, the photoswitchable DitIMI showed a large differencein insecticidal activity between the open and closed forms, in which the insecticidal activity against Aphis craccivora of the ring-closed isomers for DitIMIwas 355 times that of the corresponding ring-open isomers. This photoisomerizationcan further be translated to photomodulation of neuron membrane potentialand behavioral responses of living mosquito larvae and American cockroaches.The photomanipulation of nACh neurotransmission opens new avenuesto understanding inhibitory circuits in intact animals.