文献类型：期刊文献

英文题名：Influence of dextrins on the production of spiramycin and impurity components by Streptomyces ambofaciens

作者：Yao, Kaiya[1];Gao, Shuhong[1];Wu, Yanjie[2];Zhao, Zhen[2];Wang, Wen[2];Mao, Quangui[2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Topfond Pharmaceut Co Ltd, Zhumadian 463000, Henan, Peoples R China

年份：2018

卷号：63

期号：1

起止页码：105

外文期刊名：FOLIA MICROBIOLOGICA

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000418143600014)】；

基金：This work was supported by the National Science Foundation of China for Young Scholars (grant no. B060804) and the Open Funding Project of the State Key Laboratory of Bioreactor Engineering.

语种：英文

摘要：Spiramycin is a 16-membered macrolide antibiotic produced by Streptomyces ambofaciens and used in human medicine for the treatment of various respiratory tract and genital infections. Several impurities were detected in spiramycin-fermentation broth, especially impurities D and F, which decreased the separation-extraction yield and increased production cost. Dextrins, as the main carbon source, influence the accumulation of spiramycin and impurities. In this work, two types of dextrin from vendor Y and Z were compared to study their influences on spiramycin production. Our results showed that final spiramycin production with dextrin Z was enhanced twofold as compared with dextrin Y; however, the content of impurities F and D were higher with dextrin Z relative to dextrin Y. Several parameters (adenosine triphosphate, total sugar, reducing sugar, and reducing sugar to total sugar) were analyzed to reveal differences in the fermentation process. In vitro dextrin hydrolysis by amylase revealed structural differences in the two types of dextrin, and real-time quantitative polymerase chain reaction analyses showed that the transcription of srm7 and srm21 (involved in forosaminyl methylation) was enhanced and potentially related to the reduced formation of impurity F with dextrin Y. Furthermore, the srm20/srm33 ratio, representing flux balance of forosaminyl and mycarosyl, was similar to 1, implying that forosaminyl and mycarosyl biosynthesis were well balanced, resulting in reduced production of impurity D with dextrin Y.