文献类型：期刊文献

英文题名：Serum Metabolomic Characterization of Liver Fibrosis in Rats and Anti-Fibrotic Effects of Yin-Chen-Hao-Tang

作者：Zhang, Hongyang[1];Wang, Xiaoning[2];Hu, Ping[1];Zhou, Wenjun[2];Zhang, Min[3];Liu, Jia[2];Wang, Yuerong[1];Liu, Ping[2,4];Luo, Guoan[3,5]

机构：[1]E China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[2]Shanghai Univ Tradit Chinese Med, E Inst Shanghai Municipal Educ Comm, Shanghai 201203, Peoples R China;[3]E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China;[4]Shuguang Hosp, Inst Liver Dis, Minist Educ, Key Lab Liver & Kidney Dis, Shanghai 201203, Peoples R China;[5]Tsinghua Univ, Dept Chem, Beijing 100084, Peoples R China

年份：2016

卷号：21

期号：1

外文期刊名：MOLECULES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000369486800022)】；

基金：This research was financially supported by the National Natural Science Foundation of China (Nos. 81202493, 81273481, 81573873, 8153000502 and 81273728), the Fundamental Research Funds from the Central Universities, the National Science and Technology Major Project for Significant New Drugs Development (No. 2013ZX09507005), the Program for Budgeted Scientific Research Project of Shanghai Municipal Education Commission, China (I3YZ044), the Natural Science Foundation of Shanghai, China (14ZR1441400), and the E-institutes of Shanghai Municipal Education Commission, China (E03008).

语种：英文

外文关键词：serum metabolomics; liver fibrosis; Yin-Chen-Hao-Tang; anti-fibrotic effects; oxidative stress; lipid peroxidation; ultra-performance liquid chromatography-time-of-flight mass spectrometry; partial least squares-discriminant analysis

摘要：Yin-Chen-Hao-Tang (YCHT) is a famous Chinese medicine formula which has long been used in clinical practice for treating various liver diseases, such as liver fibrosis. However, to date, the mechanism for its anti-fibrotic effects remains unclear. In this paper, an ultra-performance liquid chromatography-time-of-flight mass spectrometry (UPLC-TOF-MS)-based metabolomic study was performed to characterize dimethylnitrosamine (DMN)-induced liver fibrosis in rats and evaluate the therapeutic effects of YCHT. Partial least squares-discriminant analysis (PLS-DA) showed that the model group was well separated from the control group, whereas the YCHT-treated group exhibited a tendency to restore to the controls. Seven significantly changed fibrosis-related metabolites, including unsaturated fatty acids and lysophosphatidylcholines (Lyso-PCs), were identified. Moreover, statistical analysis demonstrated that YCHT treatment could reverse the levels of most metabolites close to the normal levels. These results, along with histological and biochemical examinations, indicate that YCHT has anti-fibrotic effects, which may be due to the suppression of oxidative stress and resulting lipid peroxidation involved in hepatic fibrogenesis. This study offers new opportunities to improve our understanding of liver fibrosis and the anti-fibrotic mechanisms of YCHT.