文献类型：期刊文献

英文题名：Potentiation effect of HB-EGF on facilitating wound healing via 2-N, 6-O-sulfated chitosan nanoparticles modified PLGA scaffold

作者：Peng, Xiang[1,2];Yu, Yuanman[1,2];Wang, Zihao[1,2];Zhang, Xiaohui[2];Wang, Jing[1,2];Liu, Changsheng[1,2]

机构：[1]East China Univ Sci & Technol, Key Lab Ultrafine Mat, Minist Educ, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Engn Res Ctr Biomed Mat, Minist Educ, Shanghai 200237, Peoples R China

年份：2017

卷号：7

期号：68

起止页码：43161

外文期刊名：RSC ADVANCES

收录：;EI(收录号：20173704156097);WOS:【SCI-EXPANDED(收录号:WOS:000409548200057)】；

基金：The authors wish to express their gratitude for financial supports from the National Natural Science Foundation of China (No. 31330028, No. 31470923), International Cooperation Project of Shanghai Science and Technique Committee (15520711100), and the Key Project of Logistic Scientific Research of PLA (BWS13C014). This study was also supported by the 111 Project (B14018).

语种：英文

外文关键词：Scaffolds (biology) - Chitosan

摘要：Heparin-binding epidermal growth factor (HB-EGF) is of extreme importance for accelerating wound healing; however, its effective and controlled release remains to be solved. In this study, 2-N, 6-O-sulfated chitosan (26SCS), a highly sulfated polysaccharide, was utilized to fabricate delivery vehicles of HB-EGF encapsulated in PLGA nanofibers to achieve effective release. In addition, migration of Hacat cells was greatly promoted by 26SCS synergizing with HB-EGF released from the PLGA scaffold and proliferation was not affected. In a mouse excisional splinted model, a wound treated with HB-EGF loaded S-PLGA nanofibers was basically healed at 14 days. Conversely, wounds in other groups, including HB-EGF-loaded PLGA nanofibers, were not closured. The acceleration of wound healing was ascribed to the 26SCS-favored HB-EGF effect on the migration of keratinocyte which led to rapid reepithelialization. Furthermore, neovascularization was also observed in the HB-EGF loaded S-PLGA nanofiber treated wound. These data strongly proved that S-PLGA nanofibers with incorporated HB-EGF may provide a promising way for rapid regeneration of the cutaneous wounds.