文献类型：期刊文献

中文题名：发酵液中洛伐他汀及其开环酸的HPLC测定

英文题名：Determination of Lovastatin and Lovastatin Acid in Fermentation Broth by HPLC

作者：冯建立[1];许振良[1];王学军[1];杨座国[1]

机构：[1]华东理工大学化学工程研究所,上海200237

年份：2006

卷号：37

期号：7

起止页码：494

中文期刊名：中国医药工业杂志

外文期刊名：Chinese Journal of Pharmaceuticals

收录：CSTPCD;;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：洛伐他汀;发酵液;高效液相色谱;测定

外文关键词：lovastatin; fermentation broth; HPLC; determination

摘要：建立了HPLC法同时测定发酵液中洛伐他汀及其开环酸的含量。采用C8柱,流动相为10mmol/L磷酸-乙腈(40∶60),检测波长238nm。洛伐他汀及其开环酸分别在0.012～0.096mg/ml和0.01～0.08mg/ml范围内线性关系良好,平均回收率分别为99.8%和98.7%。
A HPLC method was established for the determination of lovastatin and lovastatin acid in the fermentation broth. A Ca column was used with the mobile phase of 10mmol/L phosphoric acid-acetonitrile （40 ： 60）, at the detection wavelength of 238nm. The calibration curves of lovastatin and lovastatin acid were linear in the ranges of 0.012 - 0.096mg/ml and 0.01 - 0.08mg/ml, respectively. The average recoveries were 99.8% and 98.7%, respectively.