文献类型：期刊文献

中文题名：Brocaeloid D,a novel compound isolated from a wheat pathogenic fungus, Microdochium majus 99049

作者：Jingyu Zhang[1];Zhenzhen Wang[1];Zhijun Song[2,5];Loganathan Karthik[1];Chengjian Hou[1];Guoliang Zhu[1];Lan Jiang[1];Jianying Han[2,5];Rong Ma[1];Li Li[3];Lixin Zhang[1,2];Xueting Liu[1];Tom Hsiang[4]

机构：[1]State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai,200237,China;[2]Key Laboratory of Pathogenic Microbiology and Immunology,Institute of Microbiology,Chinese Academy of Sciences,Beijing,100101,China;[3]Department of Medicinal Chemistry,Institute of Materia Medica,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing,100050,China;[4]School of Environmental Sciences,University of Guelph,50 Stone Road East,Guelph,Ontario,N1G 2W1,Canada;[5]University of Chinese Academy of Sciences,Beijing,100049,China

年份：2019

卷号：4

期号：4

起止页码：173

中文期刊名：Synthetic and Systems Biotechnology

外文期刊名：合成和系统生物技术（英文）

收录：Scopus

基金：This work was partially supported by the grants from the National Natural Science Foundation of China(31430002,81573341,21877038,31720103901,31320103911);Taishan Scholarship,Open Project Funding of the State Key Laboratory of Bioreactor Engineering,the 111 Project(B18022);National Key R&D Program of China 2017YFE0108200;the Fundamental Research Funds for the Central Universities(22221818014).

语种：英文

中文关键词：Wheat pathogenic fungus;Anticancer;Antibacterial;Natural product

摘要：Microbes serve as the most important resource for drug discovery.During our screening for bioactive compounds from our natural products library,a pathogenic fungus,Microdochium majus strain 99049,from wheat was selected for further investigation.A new alkaloid named brocaeloid D(1),together with six previously characterized compounds(2–7)were identified.Compound 1 belongs to 4-oxoquinoline with C-2 reversed prenylation and a succinimide substructure.All the structures of these newly isolated compounds were determined by different means in spectroscopic experiments.The absolute configurations of 1 was further deduced from comparison of its CD spectrum with that of known compound 2.The bioactivities of these identified compounds were evaluated against several pathogenic microorganisms and cancer cell lines.Compounds 1–5 showed activity against HUH-7 human hepatoma cells with IC50 values of 80μg/mL.Compound 6 showed mild activity against HeLa cells(IC50=51.9μg/mL),weak anti-MTB activity(MIC=80μg/mL),and moderate anti-MRSA activity(MIC=25μg/mL),and compound 7 showed weak anti-MRSA activity(MIC=100μg/mL).