文献类型：期刊文献

英文题名：Dendritic Mesoporous Silica Nanoparticles with Abundant Ti4+ for Phosphopeptide Enrichment from Cancer Cells with 96% Specificity

作者：Hong, Yayun[1];Yao, Yating[2];Zhao, Hongli[1];Sheng, Qanying[1];Ye, Mingliang[2];Yu, Chengzhong[3];Lan, Minbo[1]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Natl Chromatog R&A Ctr, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China;[3]Univ Queensland, Australian Inst Bioengn & Nanotechnol, Brisbane, Qld 4072, Australia

年份：2018

卷号：90

期号：12

起止页码：7617

外文期刊名：ANALYTICAL CHEMISTRY

收录：;EI(收录号：20182305268265);WOS:【SCI-EXPANDED(收录号:WOS:000436028800072)】；

基金：This research was financially supported by the Science and Technology Commission of Shanghai Municipality (STCSM, No. 16520710800), the Fundamental Research Funds for the Central Universities (No. 222201817022), the Natural Science Foundation of Shanghai (No. 16ZR1407600), and the Shanghai Sailing Program (No. 16YF1402400). C.Y. acknowledges the support from Australian Research Council, Australian National Fabrication Facility at The University of Queensland.

语种：英文

外文关键词：Silica nanoparticles - TiO2 nanoparticles - Chelation - Mesoporous materials - Lanthanum compounds - Pore structure

摘要：Selective enrichment and sensitive detection of phosphopeptides are of great significance in many bioapplications. In this work, dendritic mesoporous silica nanoparticles modified with polydopamine and chelated Ti4+ (denoted DMSNs@PDA-Ti4+) were developed to improve the enrichment selectivity of phosphopeptides. The unique central-radial pore structures endowed DMSNs@PDA-Ti4+ with a high surface area (362 m(2) g(-1)), a large pore volume (1.37 cm(3) g(-1)), and a high amount of chelated Ti4+ (75 mu g mg(-1)). Compared with conventional mesoporous silica-based materials with the same functionalization (denoted mSiO(2)@PDA-Ti4+) and commercial TiO2, DMSNs@PDA-Ti4+ showed better selectivity and a lower detection limit (0.2 fmol/mu L). Moreover, 2422 unique phosphopeptides were identified from HeLa cell extracts with a high specificity (>95%) enabled by DMSNs@PDA-Ti4+, better than those in previous reports.