文献类型：期刊文献

英文题名：Programmable Electro-Assembly of Collagen: Constructing Porous Janus Films with Customized Dual Signals for Immunomodulation and Tissue Regeneration in Periodontitis Treatment

作者：Lei, Miao[1];Wan, Haoran[1];Song, Jia[2,3];Lu, Yanhui[2,3];Chang, Ronghang[1];Wang, Honglei[1];Zhou, Hang[1];Zhang, Xuehui[2,3];Liu, Changsheng[1];Qu, Xue[1,4,5]

机构：[1]East China Univ Sci & Technol, Frontiers Sci Ctr Materiobiol & Dynam Chem, Sch Mat Sci & Engn, Key Lab Ultrafine Mat,Minist Educ, Shanghai 200237, Peoples R China;[2]Peking Univ, Sch & Hosp Stomatol, Dept Dent Mat, Beijing 100081, Peoples R China;[3]Peking Univ, Sch & Hosp Stomatol, Dent Med Devices Testing Ctr, NMPA Key Lab Dent Mat, Beijing 100081, Peoples R China;[4]East China Univ Sci & Technol, Shanghai Frontier Sci Res Base Optogenet Tech Cell, Shanghai 200237, Peoples R China;[5]Shanghai Univ, Wenzhou Inst, Wenzhou 325000, Peoples R China

年份：2024

卷号：11

期号：13

外文期刊名：ADVANCED SCIENCE

收录：;EI(收录号：20240215341313);WOS:【SCI-EXPANDED(收录号:WOS:001142154700001)】；

基金：M.L., H.W., J.S., and Y.L. contributed equally to this work. Support from the National Key Research and Development Program of China (2021YFB3800800), the National Natural Science Foundation of China (31922041, 32171341, 32301113, 82022016, 52273258, 82301076), the Science and Technology Innovation Project and Excellent Academic Leader Project of Shanghai Science and Technology Committee (21S31901500, 21XD1421100), the National Postdoctoral Program for Innovative Talents (BX20230122), the Shanghai Sailing Program (23YF1409700), the Beijing Natural Science Foundation (7222226) and the China Postdoctoral Science Foundation (2022M721136, 2022M720008).

语种：英文

外文关键词：collagen; electro-assembly; immunomodulatory activity; Janus porous structure; periodontitis treatment

摘要：Currently available guided bone regeneration (GBR) films lack active immunomodulation and sufficient osteogenic ability- in the treatment of periodontitis, leading to unsatisfactory treatment outcomes. Challenges remain in developing simple, rapid, and programmable manufacturing methods for constructing bioactive GBR films with tailored biofunctional compositions and microstructures. Herein, the controlled electroassembly of collagen under the salt effect is reported, which enables the construction of porous films with precisely tunable porous structures (i.e., porosity and pore size). In particular, bioactive salt species such as the anti-inflammatory drug diclofenac sodium (DS) can induce and customize porous structures while enabling the loading of bioactive salts and their gradual release. Sequential electro-assembly under pre-programmed salt conditions enables the manufacture of a Janus composite film with a dense and DS-containing porous layer capable of multiple functions in periodontitis treatment, which provides mechanical support, guides fibrous tissue growth, and acts as a barrier preventing its penetration into bone defects. The DS-containing porous layer delivers dual bio-signals through its morphology and the released DS, inhibiting inflammation and promoting osteogenesis. Overall, this study demonstrates the potential of electrofabrication as a customized manufacturing platform for the programmable assembly of collagen for tailored functions to adapt to specific needs in regenerative medicine. A drug-modulated electro-assembly of collagen GBR film, offering customized porous microstructures (i.e., porosity and pore size) and therapeutic salt component is reported here. This water-based fabrication method is convenient, fast, and allows stepwise creation of bioactive GBR film with dense/porous Janus layers. The coupling of microstructures and specific anti-inflammatory components within the Janus film demonstrates a synergistic potential for inflammation suppression and alveolar bone regeneration promotion.image