文献类型：期刊文献

英文题名：A new thermostable β-glucosidase mined from Dictyoglomus thermophilum: Properties and performance in octyl glucoside synthesis at high temperatures

作者：Zou, Zheng-Zheng[1]; Yu, Hui-Lei[1]; Li, Chun-Xiu[1]; Zhou, Xin-Wen[2]; Hayashi, Chihiro[3]; Sun, Jie[1]; Liu, Bao-Hong[2]; Imanaka, Tadeyuki[1,3]; Xu, Jian-He[1]

机构：[1] Laboratory of Biocatalysis and Synthetic Biotechnology, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China; [2] Department of Chemistry and Institute of Biomedical Sciences, Fudan University, Shanghai 200433, China; [3] Department of Biotechnology, College of Life Sciences, Ritsumeikan University, Shiga 525-8577, Japan

年份：2012

卷号：118

起止页码：425

外文期刊名：Bioresource Technology

收录：EI(收录号：20122815234420)

语种：英文

外文关键词：Cloning - Glucose - Solutions

摘要：A new β-glucosidase (DtGH) representing 40% identity with an apple seed glycosidase (ASG) was cloned from Dictyoglomus thermophilum. DtGH showed extremely high thermostability in aqueous solution, with half-lives of 533, 44, and 5. h measured at 70, 80 and 90. °C, respectively. Therefore it was used for direct glycosylation of n-octanol at 70. °C instead of 50. °C as usually. As a result, the glucose based conversion was increased by 27%, but the time spent to reach equilibrium was decreased from 7 d to 3 d. This enzyme also exhibited excellent stability under the reaction environment, retaining 70-80% of its initial activity after 7 d of incubation at 70. °C in either 1.7. M glucose solution or octanol-aqueous (85:15, v/v) system. It could retain part of synthetic activity even in boiling water. Owing to the strong glucose-tolerance and extremely high thermostability, DtGH should be promising for various glucosides synthesis. ? 2012.