文献类型：期刊文献

中文题名：油水乳化体系中分枝杆菌转化植物甾醇产9α-羟基雄甾烯酮工艺研究

英文题名：Biotransformation of Phytosterols to 9α-Hydroxyandrostenedione by Mycobacteriumsp.in Oil-Water Emulsion System

作者：高兴强[1];冯建勋[1];花强[1];王学东[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2014

卷号：40

期号：4

起止页码：433

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

基金：国家自然科学基金(21276083)

语种：中文

中文关键词：分枝杆菌;植物甾醇;9-羟基雄甾-4-烯-3,17-二酮;油水乳化体系

外文关键词：Mycobacterium; phytosterols; 9-hydroxyandrost-4-ene 3, 17-dione; oil-water emulsion system

摘要：为了提高分枝杆菌转化植物甾醇产9α-羟基雄甾-4-烯-3,17-酮(9-OH-AD)的转化效率,建立了油水乳化体系。结果表明:机械搅拌提供剪切力和加入吐温80作为分散剂是形成稳定乳化体系的必要因素,且油的比例须随甾醇投料质量浓度的增加而增加。在油水乳化体系中,细胞均匀分散,生物量增加,植物甾醇投料浓度和转化效率均得到提高;植物甾醇投料质量浓度可达20g/L,9-OH-AD摩尔得率达到64.06%,产率为1.28g/(L·d)。转化终止后通过离心进行相分离,可以去除大部分存在于油相中的副产物,使产物纯度从66%提高到90%。
A simple oil-water emulsion system was developed for highly efficient biotransformation of phytosterols to 9α-hydroxyandrost-4-ene 3, 17-dione （9-OH-AD）. Results indicated that mechanical shearing force generating shear force and addition of Tween 80 as dispersant were the essential factors to form a stable emulsion system. And the ratio of cereal oil was related to the effective load of system for phytosterols concentration. In the emulsion system, a higher biomass was got at an evenly dispersed state. Application of the emulsion system accelerated bio＇transformation process at higher phyosterols concentration of 20 g/I. phytosterols with 64.06% molar yield and 1.28 g/（L·d） productivity of 9-OH AD. After biotransformation, the major by-products partitioned in the oil phase would be removed with oil, and the purity of 9 OH-AD by the chromatography area normalizing increased from 66% to 90%.