文献类型：期刊文献

英文题名：Immune responses evoked by infection with Vibrio anguillarum in zebrafish bath-vaccinated with a live attenuated strain

作者：Zhang, Zhihui[1];Wu, Haizhen[1];Xiao, Jingfan[1];Wang, Qiyao[1];Liu, Qin[1];Zhang, Yuanxing[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2013

卷号：154

期号：3-4

起止页码：138

外文期刊名：VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000322090500007)】；

基金：This work was supported by grants from the National Natural Science Foundation of China (No. 31001121), the Fundamental Research Funds for the Central Universities (No. WF0914039), Program for New Century Excellent Talents in University (No. Ncet-09-0344) and the Shanghai Leading Academic Discipline Project 278 (No. B505).

语种：英文

外文关键词：Immune response; Bath-vaccination; Zebrafish; Live attenuated vaccine; Infection

摘要：Live attenuated vaccines are a promising application to control bacterial fish diseases. A live attenuated Vibrio anguillarum vaccine candidate was established in our laboratory to protect fish against vibriosis. To elucidate the mechanism of immunoprotection, it is necessary to compare the different immune responses to infection between vaccinated and non-vaccinated fish. In this study, the expression levels of pathogen-specific antibodies and immune-related genes upon challenge at 28 days post-vaccination were compared between vaccinated and non-vaccinated zebrafish. In the results, the specific antibody levels against virulent V. anguillarum in the vaccinated group did not rise significantly following infection, which suggested that high-affinity antibodies were induced by the vaccine. In the non-vaccinated group, the specific IgM response was triggered at 3 days post-infection and showed a delayed antibody response. Meanwhile, the transcription levels of the genes encoding the pro-inflammatory cytokine IL-1 beta and the chemokine IL-8 were more highly up-regulated in non-vaccinated fish than in vaccinated fish. This suggests that the overwhelming inflammatory response trigged by infection in non-vaccinated zebrafish was controlled in vaccinated zebrafish. Interestingly, the expression levels of adaptive immune-related genes were increased in vaccinated fish after challenge, compared to the non-vaccinated fish. These results suggest that inoculation with the live attenuated vaccine triggered protection by curbing inflammation and strengthening the adaptive immune response. (C) 2013 Elsevier B.V. All rights reserved.