文献类型：期刊文献

英文题名：Tyrosinase inhibitory effect and inhibitory mechanism of tiliroside from raspberry

作者：Lu, Yan-hua[1];Chen, Juan[1];Wei, Dong-zhi[1];Wang, Zheng-tao[2];Tao, Xin-yi[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Univ TCM, Inst Chinese Mat Med, Shanghai 201203, Peoples R China

年份：2009

卷号：24

期号：5

起止页码：1154

外文期刊名：JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000271613300017)】；

基金：This work was supported by Shanghai Leading Academic Discipline Project (13505).

语种：英文

外文关键词：Raspberry; tiliroside; Rubus chingii hu; mushroom tyrosinase; inhibition mechanism; B16 mouse melanoma cells; melanin

摘要：Tiliroside was found to inhibit both monophenolase and diphenolase activity of mushroom tyrosinase. The lag time of tyrosine oxidation catalyzed by mushroom tyrosinase was obviously lengthened; 0.337 mM of tiliroside resulted in the lag time extension from 46.7s to 435.1s. A kinetic analysis shown that tiliroside was a competitive inhibitor for monophenolase and diphenolase with K, values of 0.052 mM and 0.26 mM, respectively. Furthermore, tiliroside showed 34.5% (p < 0.05) inhibition of intracellular tyrosinase activity and 54.1% (p < 0.05) inhibition of melanin production with low cytotoxicity on B16 mouse melanoma cells at 0.168 mM. In contrast, arbutin displayed 9.1% inhibition of cellular tyrosinase activity and 29.5% inhibition of melanin production at the same concentration. These results suggested that tiliroside was a potent tyrosinase inhibitor and might be used as a skin-whitening agent and pigmentation medicine.