成果/Result
- Simultaneous production and partitioning of heterologous polyketide and isoprenoid natural products in an Escherichia coli two-phase bioprocess被引量：67收藏
- 作者：Boghigian, Brett A. Myint, Melissa Wu, Jiequn Pfeifer, Blaine A.
- 机构： Department of Chemical and Biological Engineering; State Key Laboratory of Bioreactor Engineering
- 来源：Journal of Industrial Microbiology and Biotechnology 2011
- 关键词：Biochemistry - Bioconversion - Bioreactors - Drug products - Ketones - Lipids - Molecules
- Industrial bioprocess control and optimization in the context of systems biotechnology被引量：56收藏
- 作者：Wang, Yong Chu, Ju Zhuang, Yingping Wang, Yonghong Xia, Jianye Zhang, Siliang
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Biotechnology Advances 2009
- Alleviation of photoinhibition by calcium supplement in salt-treated Rumex leaves被引量：49收藏
- 作者：Chen, Hua-Xin Li, Peng-Min Gao, Hui-Yuan
- 机构： National Engineering Research Center for Biotechnology; College of Life Sciences; Shandong Key Laboratory of Crop Biology
- 来源：Physiologia Plantarum 2007
- 关键词：Calcium compounds - Chlorophyll - Photosynthesis - Sodium chloride
- In silico analysis and experimental improvement of taxadiene heterologous biosynthesis in Escherichia coli被引量：37收藏
- 作者：Meng, Hailin Wang, Yong Hua, Qiang Zhang, Siliang Wang, Xiaoning
- 机构： School of Bioscience and Bioengineering; Key Laboratory of Synthetic Biology; State Key Laboratory of Bioreactor Engineering
- 来源：Biotechnology and Bioprocess Engineering 2011
- 关键词：Thermodynamic properties - Chromosomes - Lipids - Escherichia coli - Biochemistry
- Construction and performance of heterologous polyketide-producing K-12- and B-derived Escherichia coli被引量：33收藏
- 作者：Wu, J. Boghigian, B.A. Myint, M. Zhang, H. Zhang, S. Pfeifer, B.A.
- 机构： State Key Laboratory of Bioreactor Engineering; Department of Chemical and Biological Engineering
- 来源：Letters in Applied Microbiology 2010
- 关键词：Biochemistry - Biosynthesis - Gene expression
- DNA shuffling of methionine adenosyltransferase gene leads to improved S-adenosyl-l-methionine production in Pichia pastoris被引量：32收藏
- 作者：Hu, Hui Qian, Jiangchao Chu, Ju Wang, Yong Zhuang, Yingping Zhang, Siliang
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Journal of Biotechnology 2009
- 关键词：Escherichia coli - Yeast - Amino acids - Genes - Enzyme activity
- Identification and purification of the main components of cellulases from a mutant strain of Trichoderma viride T 100-14被引量：30收藏
- 作者：Zhou, Jin Wang, Yong-Hong Chu, Ju Zhuang, Ying-Ping Zhang, Si-Liang Yin, Peng
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Bioresource Technology 2008
- 关键词：Cultivation - Electrophoresis - Fungi - Purification
- Purification and properties of Saccharomyces cerevisiae S-adenosylmethionine synthetase expressed in recombinant Pichia pastoris被引量：24收藏
- 作者：Zhou, Jin Chu, Ju Wang, Yong-Hong Zhang, Si-Liang Zhuang, Ying-Ping Yuan, Zhong-Yi
- 机构： State Key Laboratory of Bioreactor Engineering; National Research Center for Biotechnology
- 来源：World Journal of Microbiology and Biotechnology 2008
- 关键词：Enzyme activity - Fermentation - Gel permeation chromatography - pH effects - Purification
- Improvement of innate immune responses and defense activity in mitten crab (Eriocheir sinensis) by oral administration of β-glucan被引量：18收藏
- 作者：Zhou, Jin Wang, Yong-Hong Chu, Ju Luo, Ling-Zhi
- 机构： State Key Laboratory of Bioreactor Engineering; Shenzhen Graduate School of Tsinghua University
- 来源：Biotechnology Letters 2008
- 关键词：Dietary supplements - Superoxide dismutase - Yeast - Shellfish
- Effect of mycelial morphology on bioreactor performance and avermectin production of Streptomyces avermitilis in submerged cultivations被引量：15收藏
- 作者：Yin, Peng Wang, Yong-Hong Zhang, Si-Liang Chu, Ju Zhuang, Ying-Ping Chen, Ning Li, Xin-Feng Wu, Yan-Bin
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Journal of the Chinese Institute of Chemical Engineers 2008
- 关键词：Bioreactors - Dissolved oxygen - Pelletizing - Bacteria - Nitrogen
- Medium optimization for meilingmycin production by Streptomyces nanchangensis using response surface methodology被引量：14收藏
- 作者：Zhuang, Ying Ping Chen, Bin Chu, Ju Zhang, Siliang
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Process Biochemistry 2006
- 关键词：Food products - Oils and fats - Optimization - Sodium chloride - Statistical mechanics - Yeast
- Purification and bioactivity of exendin-4, a peptide analogue of GLP-1, expressed in Pichia pastoris被引量：14收藏
- 作者：Zhou, Jin Chu, Ju Wang, Yong-Hong Wang, Hui Zhuang, Ying-Ping Zhang, Si-Liang
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Biotechnology Letters 2008
- 关键词：Bioactivity - Bioassay - Insulin - Medical problems - Methanol - Yeast
- 温度诱导模式对重组大肠杆菌质粒拷贝数的影响被引量：14收藏
- 作者：丁满生 马文峰 郭美锦 储炬 张嗣良 龚邦强
- 机构：华东理工大学国家生化工程技术中心(上海)
- 来源：《华东理工大学学报（自然科学版）》 2006
- 关键词：温度 诱导 质粒 拷贝数 大肠杆菌
- 摘要：对于大肠杆菌温度表达系统,温度的选择和变化对外源基因的表达至关重要,最直接的因素表现在对质粒拷贝数的影响上。本文通过重组大肠杆菌DH 5α表达载脂蛋白A I-M ilano来分析不同温度诱导模式对质粒拷贝数的影响,确立了...
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- 重组毕赤酵母表达猪胰岛素前体代谢参数分析和动力学模型被引量：6收藏
- 作者：陈文 郭美锦 储炬 庄英萍 张嗣良
- 机构：华东理工大学生物反应器工程国家重点实验室国家生化工程技术研究中心
- 来源：《华东理工大学学报（自然科学版）》 2005
- 关键词：重组毕赤酵母 猪胰岛素前体 代谢参数相关分析 动力学模型
- 摘要：采用发酵过程参数相关分析理论,分析了毕赤酵母在不同发酵阶段利用不同碳源时的生理代谢参数变化特征。同时,研究了不同比生长速率下重组毕赤酵母表达猪胰岛素前体补料分批发酵过程,建立蛋白表达阶段的细胞生长非结构模型,结果表明:比...
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- Temperature-control Strategy in an Industrial Clavulanic Acid Fermentation被引量：0收藏
- 作者：FENG TAO ZHU XIAO-SHI CHU JU WANG YONG-HONG ZHANG SI-LIANG CAO BIN
- 机构：State Key Laboratory of Bioreactor Engineering;Shanxi Weiqida Pharmaceutical Co
- 来源：[1]State Key Laboratory of Bioreactor Engineering, National Engineering Research Center for Biotechnology,East China University of Science and Technology, 200237 Shanghai,China;[2]Shanxi Weiqida Pharmaceutical Co, Ltd, 037300 Datong, China; 2008
