成果/Result
- Biodegradation of Structurally Diverse Phthalate Esters by a Newly Identified Esterase with Catalytic Activity toward Di(2-ethylhexyl) Phthalate被引量：75收藏
- 作者：Huang, Han Zhang, Xiao-Yan Chen, Tian-Lei Zhao, Yu-Lian Xu, Dian-Sheng Bai, Yun-Peng
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2019
- 关键词：Gordonia polyisoprenivorans esterase bis(2-ethylhexyl) phthalate phthalate esters biodegradation
- Cross-linked enzyme-polymer conjugates with excellent stability and detergent-enhanced activity for efficient organophosphate degradation被引量：17收藏
- 作者：Cheng, Huan Zhao, Yu-Lian Luo, Xiao-Jing Xu, Dian-Sheng Cao, Xun Xu, Jian-He Dai, Qing Zhang, Xiao-Yan Ge, Jun Bai, Yun-Peng
- 机构：East China Univ Sci & Technol;Tsinghua Univ;Natl Ctr Nanosci & Technol
- 来源：BIORESOURCES AND BIOPROCESSING 2018
- 关键词：Organophosphate Organophosphate hydrolase Immobilization Biodegradation Enzyme-polymer conjugates Pluronic F127
- Efficient Degradation of Malathion in the Presence of Detergents Using an Engineered Organophosphorus Hydrolase Highly. Expressed by Pichia pastoris without Methanol Induction被引量：17收藏
- 作者：Bai, Yun-Peng Luo, Xiao-Jing Zhao, Yu-Lian Li, Chun-Xiu Xu, Dian-Sheng Xu, Jian-He
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2017
- 关键词：biodegradation Pichia pastoris organophosphorus hydrolase extracellular expression malathion
- Purification and In Situ Immobilization of Papain with Aqueous Two-Phase System被引量：15收藏
- 作者：Li, Mingliang Su, Erzheng You, Pengyong Gong, Xiangyu Sun, Ming Xu, Diansheng Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：PLOS ONE 2010
- Improving the refolding of NTA protein by urea gradient and arginine gradient size-exclusion chromatography被引量：10收藏
- 作者：Fan, Xiangdong Xu, DianSheng Lu, Bing Xia, Jie Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS 2008
- 关键词：refolding two gradients NTA protein aggregation chromatography
- Refolding and purification of rhNTA protein by chromatography被引量：3收藏
- 作者：Fan, Xiangdong Xu, DianSheng Lu, Bing Xia, Jie Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：BIOMEDICAL CHROMATOGRAPHY 2009
- 关键词：RhNTA protein refolding two gradients purification chromatography
- MMP-12蛋白包涵体复性时折叠状态核磁共振图谱与荧光光谱研究被引量：1收藏
- 作者：张琳琳 冯延叶 蓝文贤 曹春阳 徐殿胜 杨忠
- 机构：华东理工大学生物反应器工程国家重点实验室;复旦大学生命科学学院微生物学和微生物工程系;中国科学院上海有机化学研究所生命有机国家重点实验室
- 来源：《化学学报》 2012
- 关键词：核磁共振 稳态荧光 ANS荧光探针 MMP-12 复性折叠
- 摘要：MMP-12是癌症治疗药物靶标.为了更好研制新药,需要大量制备MMP-12,但MMP-12在大肠杆菌中以包涵体形式表达.因此如何优化蛋白复性过程是大量获取MMP-12蛋白的关键.采用核磁共振、稳态荧光法、外源性ANS(8...
- 下载全文在线阅读
