成果/Result
- The Important Role of Halogen Bond in Substrate Selectivity of Enzymatic Catalysis被引量：78收藏
- 作者：Jiang, Shuiqin Zhang, Lujia Cui, Dongbin Yao, Zhiqiang Gao, Bei Lin, Jinping Wei, Dongzhi
- 机构：East China Univ Sci & Technol
- 来源：SCIENTIFIC REPORTS 2016
- Structural insights into enzymatic activity and substrate specificity determination by a single amino acid in nitrilase from Syechocystis sp PCC6803被引量：60收藏
- 作者：Zhang, Lujia Yin, Bo Wang, Chao Jiang, Shuiqin Wang, Hualei Yuan, Y. Adam Wei, Dongzhi
- 机构：E China Univ Sci & Technol;Natl Univ Singapore;Natl Univ Singapore;Natl Univ Singapore
- 来源：JOURNAL OF STRUCTURAL BIOLOGY 2014
- 关键词：Crystal structure Nitrilase Syechocystis sp PCC6803 Substrate specificity determination
- A method to rationally increase protein stability based on the charge-charge interaction, with application to lipase LipK107被引量：33收藏
- 作者：Zhang, Lujia Tang, Xiaomang Cui, Dongbing Yao, Zhiqiang Gao, Bei Jiang, Shuiqin Yin, Bo Yuan, Y. Adam Wei, Dongzhi
- 机构：E China Univ Sci & Technol;Nanjing Univ Technol;Natl Univ Singapore;Natl Univ Singapore
- 来源：PROTEIN SCIENCE 2014
- 关键词：designed protein stabilization electrostatic stabilization lipase stabilization surface charge-charge interaction Lipk107
- Identification of novel thermostable taurine-pyruvate transaminase from Geobacillus thermodenitrificans for chiral amine synthesis被引量：30收藏
- 作者：Chen, Yujie Yi, Dong Jiang, Shuiqin Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2016
- 关键词：Taurine-pyruvate transaminase omega-Transaminase Biocatalysis Thermostable transaminase Chiral amine
- Switching a nitrilase from Syechocystis sp PCC6803 to a nitrile hydratase by rationally regulating reaction pathways被引量：27收藏
- 作者：Jiang, Shuiqin Zhang, Lujia Yao, Zhiqiang Gao, Bei Wang, Hualei Mao, Xiangzhao Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Ocean Univ China
- 来源：CATALYSIS SCIENCE & TECHNOLOGY 2017
- 关键词：Amides - Biochemical engineering - Enzymes
- A computational strategy for altering an enzyme in its cofactor preference to Nad(H) and/or NadP(H)被引量：23收藏
- 作者：Cui, Dongbing Zhang, Lujia Jiang, Shuiqin Yao, Zhiqiang Gao, Bei Lin, Jinping Yuan, Y. Adam Wei, Dongzhi
- 机构：E China Univ Sci & Technol;Natl Univ Singapore
- 来源：FEBS JOURNAL 2015
- 关键词：altered coenzyme specificity molecular dynamics simulation rational computational design site-directed mutagenesis structure stability prediction
- Recent research advancements on regioselective nitrilase: fundamental and applicative aspects被引量：19收藏
- 作者：Chen, Zhi Zhao, Jiang Jiang, Shuiqing Wei, Dongzhi
- 机构：East China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2019
- 关键词：Biocatalysis Regioselective nitrilase Regioselectivity Molecular modifications Industrial applications
- Significantly enhancing the stereoselectivity of a regioselective nitrilase for the production of (S)-3-cyano-5-methylhexanoic acid using an MM/PBSA method被引量：11收藏
- 作者：Chen, Zhi Wang, Hualei Yang, Lin Jiang, Shuiqing Wei, Dongzhi
- 机构：East China Univ Sci & Technol
- 来源：CHEMICAL COMMUNICATIONS 2021
- 关键词：Binding energy - Molecular dynamics - Free energy - Hydrogen bonds
- Rational engineering of Acinetobacter tandoii glutamate dehydrogenase for asymmetric synthesis of l-homoalanine through biocatalytic cascades被引量：11收藏
- 作者：Wang, Liuzhu Diao, Shiqing Sun, Yangyang Jiang, Shuiqin Liu, Yan Wang, Hualei Wei, Dongzhi
- 机构：East China Univ Sci & Technol
- 来源：CATALYSIS SCIENCE & TECHNOLOGY 2021
- 关键词：Amino acids - Substrates - Catalyst activity - Efficiency - Mass transfer - Hydrophobicity
- Switching the regioselectivity of two nitrilases toward succinonitrile by mutating the active center pocket key residues through a semi-rational engineering被引量：10收藏
- 作者：Chen, Zhi Jiang, Shuiqing Wang, Hualei Wang, Liuzhu Wei, Dongzhi
- 机构：East China Univ Sci & Technol
- 来源：CHEMICAL COMMUNICATIONS 2019
- Energy and conformation determine the enantioselectivity of enzyme被引量：8收藏
- 作者：Gao, Bei Jiang, Shuiqin Wang, Liuzhu Zhang, Lujia Wei, Dongzhi
- 机构：East China Univ Sci & Technol;East China Normal Univ;NYU Shanghai
- 来源：BIOCHEMICAL ENGINEERING JOURNAL 2018
- 关键词：Chiral selectivity Free energy Structural conformation Rational design Lipase
- Switching the secondary and natural activity of Nitrilase from Acidovorax facilis 72 W for the efficient production of 2-picolinamide被引量：7收藏
- 作者：Wang, Liuzhu Jiang, Shuiqin Sun, Yangyang Yang, Zeyu Chen, Zhi Wang, Hualei Wei, Dongzhi
- 机构：East China Univ Sci & Technol
- 来源：BIOTECHNOLOGY LETTERS 2021
- 关键词：Biocatalyst Catalytic Promiscuity Nitrilase Protein Engineering 2-Picolinamide
- Structural insights into substrate and coenzyme preference by SDR family protein Gox2253 from Gluconobater oxydans被引量：7收藏
- 作者：Yin, Bo Cui, Dongbing Zhang, Lujia Jiang, Shuiqin Machida, Satoru Yuan, Y. Adam Wei, Dongzhi
- 机构：E China Univ Sci & Technol;Natl Univ Singapore;Natl Univ Singapore;Natl Univ Singapore Suzhou
- 来源：PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS 2014
- 关键词：crystal structure short-chain dehydrogenases reductases G oxydans Gox2253 substrate selection coenzyme preference
- Rational hinge engineering of carboxylic acid reductase from Mycobacterium smegmatis enhances its catalytic efficiency in biocatalysis被引量：6收藏
- 作者：Wang, Liuzhu Sun, Yangyang Diao, Shiqing Jiang, Shuiqin Wang, Hualei Wei, Dongzhi
- 机构：East China Univ Sci & Technol
- 来源：BIOTECHNOLOGY JOURNAL 2022
- 关键词：carboxylic acid reductases hinge region protein engineering rational design virtual mutation
- 酶及其生物催化技术的研究与应用被引量：2收藏
- 作者：杨林 陈志 姜水琴 魏东芝
- 机构：华东理工大学生物反应器工程国家重点实验室鲁华生物技术研究所
- 来源：《生物产业技术》 2019
- 关键词：酶 生物催化 选择性 精细大宗化学品 高附加值医药中间体
- 摘要：与传统化学催化工艺相比,基于酶的生物催化工艺具有反应条件温和、环境友好、操作简便、立体选择性优良等优势。当前已有多个生物催化工艺应用于生产精细大宗化学品和高附加值医药中间体,此外在食品、化妆品等领域也有广泛的应用。主要综...
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- 基于QMMM计算的腈水解酶主/副产物精准切换被引量：0收藏
- 作者：姜水琴 张鲁嘉 魏东芝
- 机构：华东理工大学生物工程学院生物反应器工程国家重点实验室鲁华生物技术研究所
- 来源：《第十一届中国酶工程学术研讨会》 2017
- 摘要：腈水解酶(EC 3.5.5.1)直接将腈转化成相应的羧酸和NH3,因其高化学特异性和对映体选择性,在精细化学品和药物中间体生产中已经被广泛认为化学催化剂的优良替代品,受到越来越多关注。研究发现腈水解酶能够催化底物腈生成主...
- 定制酶分子机器/细胞工厂,引领生物制造产业未来——2017年第十一届中国酶工程学术研讨会杜邦-杰能科中国酶工程杰出贡献奖获得者特邀报告被引量：0收藏
- 作者：姜水琴 魏东芝
- 机构：华东理工大学生物工程学院生物反应器工程国家重点实验室鲁华生物技术研究所
- 来源：《生物工程学报》 2018
- 关键词：酶分子机器 细胞工厂 生物制造 产业未来 催化剂定制
- 摘要：工业酶制剂研发与应用已经渗透到各大工业领域,但中国作为用酶大国、产酶小国面临重大挑战,鉴于以化学催化为核心的基础物质加工业面临资源、能源和环境三大危机,酶工程与生物催化已被列入许多国家的科技与产业发展战略,应用高效、清洁...
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- Crius: A novel fragment-based algorithm of de novo substrate prediction for enzymes被引量：0收藏
- 作者：Yao, Zhiqiang Jiang, Shuiqin Zhang, Lujia Gao, Bei He, Xiao Zhang, John Z. H. Wei, Dongzhi
- 机构：East China Univ Sci & Technol;East China Normal Univ;NYU Shanghai
- 来源：PROTEIN SCIENCE 2018
- 关键词：substrate prediction enzyme selectivity substrate specificity fragment-based algorithm
- 基于QMMM计算的腈水解酶主/副产物精准切换被引量：0收藏
- 作者：姜水琴 张鲁嘉 魏东芝
- 机构：华东理工大学生物工程学院生物反应器工程国家重点实验室鲁华生物技术研究所
- 来源：《第十一届中国酶工程学术研讨会》 2017
- 关键词：腈水解酶 催化作用 羧酸主产物 酰胺副产物 精准切换 QMMM计算
- 摘要：腈水解酶(EC 3.5.5.1)直接将腈转化成相应的羧酸和NH3,因其高化学特异性和对映体选择性,在精细化学品和药物中间体生产中已经被广泛认为化学催化剂的优良替代品,受到越来越多关注。研究发现腈水解酶能够催化底物腈生成主...
